正交设计优选调胃颗粒挥发油提取工艺.docVIP

正交设计优选调胃颗粒挥发油提取工艺 　　[摘要] 目的:研究调胃颗粒挥发油的提取方法。方法:采用正交设计L9(34)方法,从药材粉碎度(A)、加水量(B)及提油时间(C)三个方面考察挥发油得量,筛选提取条件。结果:最佳提油方案为A1B2C2,即白术、木香直径小于1 cm、加水量8倍、提油时间6 h;影响因素强弱依次为ABC,其中,饮片规格(药材粉碎度)对挥发油收率的影响有统计学意义(P0.05)。结论:采取正交设计研究挥发油提取方法简单合理、工艺可行。 　　[关键词] 调胃颗粒;挥发油;中药制药工艺;正交设计;提取工艺 　　[中图分类号]R284.2 [文献标识码] A[文章编号] 1673-7210(2009)08(a)-078-02 　　 　　The optimization of the extraction process for volatile oil of Tiaowei Granules by orthogonal design 　　SUN Wenge, ZHENG Qian, ZHANG Guixia 　　(The Traditional Chinese Medicine Hospital of Shijiazhuang City, Shijiazhuang 050051, China) 　　[Abstract] Objective: To study on the extraction process for volatile oil of Tiaowei Granules. Methods: By orthogonal design L9(34) method, the degree of grinding for medicinal herbs(A), water consumption(B), reflux time(C) were all used as indication to optimize the extraction process for volatile oil. Results: The best condition of extraction for volatile oil was A1B2C2, namely the diameter of medicinal herbs was smaller than 1 cm pieces for Rhizoma Atractylodis Macrocephalae and Radix Aucklandiae, the proportion of solid∶liquid was 1∶8, and the reflux time is 6 hours. The impact sequence was ABC, and the diameter of medicinal herbs had the most important influence on the extraction process for volatile oil (P0.05). Conclusion: The technology of the extraction process for volatile oil is simple, reasonable and practical by orthogonal intersection analysis. 　　[Key words] Tiaowei Granules; Volatile oil, Chinese traditional medicine preparation procedure; Orthogonal design; Extraction process 　　 　　调胃颗粒是临床上已应用多年的医院制剂,由白芍、党参、白术、木香、砂仁、桂枝、陈皮、茯苓、厚朴等17味中药组成,具有舒肝健脾、顺气开郁之功效,临床上用于治疗功能性消化不良、食道炎、胃炎、结肠炎等症。方中白术、木香、砂仁、桂枝、陈皮5味药挥发油含量较多,具有较强的药效学活性[1]。5味药配伍的提油方法少见文献报道,笔者参阅文献[2-9]中的提取方法对其进行了研究,现报道如下: 　　1 试验材料 　　调胃颗粒(批号石家庄市中医院制剂室);白术(批号,木香(批号,砂仁(批号,桂枝(批号,陈皮(批为,均购自安国市神禾中药有限分司;所用试剂均为分析纯。 　　2 方法与结果 　　2.1 单因素分析 　　2.1.1 药材粉碎度白术、木香、砂仁、桂枝、陈皮5味中药饮片的规格通常为砂仁去皮酌碎,桂枝1 c