玉屏风颗粒对过敏性鼻炎大鼠免疫因子影响.docVIP

玉屏风颗粒对过敏性鼻炎大鼠免疫因子影响 　　[摘要]通过免疫因子水平变化考察玉屏风颗粒（YPF）对卵蛋白（OVA）致大鼠过敏性鼻炎（AR）模型的治疗作用及机制研究。通过采用OVA 0.3 mg，Al（OH）3 30 mg及生理盐水l mL混合后腹腔注射，进行初次免疫，第15天鼻孔给予4%OVA 200 μg（50 μL）滴鼻进行加强免疫制作过敏性鼻炎模型，60只雄性SD大鼠随机分成正常组、模型组、受试药玉屏风颗粒3个剂量组2.7，1.35，0.68 g?kg-1和阳性对照药鼻炎康0.4 g?kg-1，灌胃给药14 d后各组大鼠腹主动脉取血分离血清，放免法检测血清组胺（HIS）、免疫球蛋白E（IgE）、白介素-4（IL-4）、肿瘤坏死因子-α（TNF-α）的含量，取鼻组织于10%甲醛中固定并检测鼻黏膜组织形态学变化。结果显示，玉屏风颗粒高、中剂量组大鼠血清HIS，IgE，IL-4，TNF-α水平下降明显，具有统计学意义（P0.05，P0.01），鼻黏膜上皮细胞有轻度变性，少量炎性细胞浸润，坏死不明显。推断玉屏风颗粒改善过敏性鼻炎大鼠鼻组织病理变化，减轻OVA致AR过程中免疫因子的生成和释放，可能是治疗过敏性鼻炎的重要机制。 　　[关键词]玉屏风颗粒；过敏性鼻炎；组胺；免疫球蛋白E；肿瘤坏死因子；白细胞介素4 　　[Abstract]To investigate the effects of Yupingfeng granule （YPF） on immune factors of the rats with allergic rhinitis （AR） induced by ovalbumin（OVA）. OVA 0.3 mg， Al（OH）3 30 mg and saline 1 mL were mixed and intraperitoneally injected for the initial immunization， 4% OVA 200 μg （50 μL） was given to the nose on the 15th day for the second immunization to establish the allergic rhinitis model. Sixty male SD rats were randomly divided into allergic rhinitis（AR） model group， Yupingfeng granule three dose （2.7，1.35，0.68 g?kg-1） groups， control drug Biyankang （0.4 g?kg-1） and normal control group. After 14 days， efforts were made to collect blood from abdominal aorta， and take nasopharynx tissues and fasten them into 10% formaldehyde for a pathological examination. The levels of HIS， IgE， IL-4 and TNF-α in serum were examined by radioimmunoassay， and nasal mucosa tissues were examined by HE staining. According to the results， the levels of HIS， IgE， IL-4 and TNF-α in serum of Yupingfeng granule groups were significantly lower than that of AR model group （P0.05， P0.01）. Nasal mucosa tissues showed slight morphological changes and inflammatory cell infiltration， with unobvious necrosis. Yupingfeng granule can improve the pathological changes of nasal mucosa tissues， and reduce the production and release of immune factors during allergic rhinitis （AR） process in vivo by OVA， which may be the important curative mechanis