游泳水环境对大鼠海马活性氧HPA轴激素影响研究.docVIP

游泳水环境对大鼠海马活性氧HPA轴激素影响研究 　　摘要：目的：探索海马、下丘脑-垂体-肾上腺皮质（HPA）轴机能对训练及水环境的反应。 　　方法：SD大鼠90%乳酸无氧阈强度负重游泳训练7周，大鼠力竭运动后即刻、力竭游泳后1小时及力竭游泳后1小时断头处死取样；另以不游泳大鼠浸水作为对照，每次浸水时间及最后取样时间与训练大鼠相一致。采用顺磁共振法测定海马羟自由基，放免法测定HPA轴激素。实验考虑了生物日节律的影响。 　　结果：大鼠力竭后即刻下丘脑促肾上腺皮质激素释放激素（CRH）显著性高于浸水即刻；训练大鼠下丘脑皮质酮整体显著性高于浸水大鼠。力竭游泳后1小时海马羟自由基和下丘脑CRH显著性低于力竭后即刻和力竭后24小时；力竭后1小时血清皮质酮显著性高于力竭后24小时；浸水后1小时血清皮质酮也显著性高于浸水后24小时。下丘脑CRH和皮质酮受到游泳和浸水双重交互作用（p0.05）；另外，取样的日时间点与下丘脑CRH、血清和下丘脑皮质酮、海马羟自由基有显著性相关，海马羟自由基与血清皮质酮呈显著负相关。游泳和浸水干扰日节律与HPA轴激素的相关关系。 　　结论：长期游泳训练在略低于无氧阈强度力竭运动，产生延迟性应激反应，水环境也产生类似的反应，可能是包括海马羟自由基在内的海马信号的调节作用（但不仅限于羟自由基）；游泳训练及水环境在一定程度上干扰HPA轴激素的节律性分泌；游泳运动中水环境对时HPA轴激素的存在交互影响，水环境对HPA轴激素的分泌一定程度上被运动掩盖。 　　关键词：运动；内分泌；氧化还原；环境；应激 　　中图分类号：G804文献标识码：A文章编号：1004-4590(2008)01-0080-05? 　　 　　Abstract: 　　AIM: The aim of current study is to research the response of long time training and immersion on hippocampus hydroxyl radical and hypothalamo-pituitary-adrenal axis hormones. 　　Methods: Sprague-Dawley rats were forced swimming burdened with at approximately 90% of the weight corresponding to the lactate concentration threshold for 7 weeks. Samples were obtained immediately, 1hr after and 24hr after an exhaustion test of grouped rats, respectively. In contrast, rats were barely immersed in water coincided with time of rats swim training, and also coincided with time of execution of swim training rats after the last immersion. Hydroxyl radicals in hippocampus were determined with electron paramagnetic resonance spectroscopy. Radioimmunoassay in detect the hormones. Rhythm of the daytime was considered. Double-blind method was used in the whole research. 　　 Results: Corticotrophin-releasing hormone (CRH) in hypothalamus of training group right after exhaustion was significantly higher than the immersion rats at the same time. Taken together of the whole three training groups, the corticosterone in hypothalamus were significantly higher than the immersion rats. The hydroxyl radical in hippocampus and the CRH in hypothalamus of the 1hr after e