瞬时外向钾电流在糖尿病心肌病电重构中作用.docVIP

瞬时外向钾电流在糖尿病心肌病电重构中作用 　　[摘要] 目的 探讨糖尿病心肌病（DCM）大鼠心室肌细胞瞬时外向钾电流（Ito）的变化，了解其在电重构中的作用。方法 选用20只健康成年SD大鼠，随机分为对照组（n=10）和DCM组（n=10），并通过病理组织学证实为DCM心肌病理学改变。分别对两组大鼠采用改进的耐钙成年大鼠急性酶分离方法分离心肌细胞，运用膜片钳技术以全细胞模式分别记录两组大鼠心室肌单细胞的Ito和膜电容，并分析比较两组大鼠心室肌Ito的变化。 结果 与对照组比较，DCM组大鼠左心室心肌细胞的Ito电流密度显著低于对照组[+70 mV时，（16.80±9.10） pA/pF vs （36.25±5.20） pA/pF]（P0.05），DCM组的Ito的I-V曲线明显较对照组下移。 结论 DCM的Ito数量明显减少，在DCM心肌电重构中起着重要作用。Ito的减少及Ito通道的分布密度不同，可导致动作电位时程与有效不应期发生变化，可能与临床严重心律失常的发生有关。 　　[关键词] 糖尿病心肌病；电重构；膜片钳；瞬时外向钾电流 　　[中图分类号] R587.2 [文献标识码] A [文章编号] 1674-4721（2014）09（a）-0004-05 　　Effect of transient outward potassium current on ventricular electrophysiological remodeling in diabetic cardiomyopathy 　　LIU Xing1 ZHANG Liang-sheng2 ZHU Bo1 ZHA Ke-lan1 ZHANG Xue-mei1 YANG You1 FAN Zhon-cai1▲ 　　1.Department of Cardiology，the Affiliated Hospital of Luzhou Medical College，Luzhou 646000，China；2.Department of Cardiology，People′s Hospital of Yongcheng City in Henan Province，Yongcheng 476600，China 　　[Abstract] Objective To investigate the changes of the transient outward potassium current （Ito） on ventricular myocardium in diabetic cardiomyopathy （DCM） rat and to explore the meaning of theses changes for ventricular electrophysiological remodeling. Methods 20 healthy adult Spprague-dawley rats were selected and randomly divided into control group （n=10） and DCM group （n=10）.The myocardial pathological alterations of DCM were identified in DCM group by histopathologic test，and cardiomyocytes in both groups were separated by advanced calcium-tolerant separation method.Whole-cell path clamp technique was used to record the unicellular changes of Ito and membrane capacitance in left ventricular myocytes for DCM and control group respectively.All data were analysed and compaired by software. Results Compared with control group，the Ito density on left ventricular myocytes was significantly lower in DCM group than that of the control group [（16.80±9.10） pA/pF vs （36.25±5.20） pA/pF，at +70 mV]（P0.05），and the I-V curve of Ito