艾灸对aa大鼠“足三里”穴位局部与氧化磷酸化相关基因表达的影响-针灸推拿学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 - - PAGE 10 - Results 1.498 different expressed genes (DEGs) were detected after mild moxibustion, 255 DEGs belong to up-regulated genes and 243 DEGs belong to down-regulated genes. 937 DEGs were detected after moxibustion with monkshood cake insulation, 355 DEGs belong to up-regulated genes and 602 DEGs belong to down-regulated genes. 106 co-expression DEGs were detected, 44 co-expression DEGs belong to up-regulated genes and 62 co-expression DEGs belong to down-regulated genes. 106 co-expression DEGs were obviously took part in 27pathway（P＜0.001）, Cox7a2, Atp6v0d2 and Atp5j were took part in the pathway oxidative phosphorylation. We use foldchange≥2.0 |Score(d)|≥2 and foldchange≤0.5 |Score(d)|≥2 as as our standard to select up-regulated genes and down-regulated genes. 2. The result of biology-repeated experiment as follow: Compared to those in control group, the expression of Cox7a2, Atp6v0d2 and Atp5j in model group were significantly down-expressed（P＜0.05）. Compared to the model group, both of mild moxibustion group and moxibustion with monkshood cake insulation group increased expression of Cox7a2, Atp6v0d2 and Atp5j（P＜0.05）, the results are consistent with the results of gene chip. Furthermore, moxibustion with monkshood cake insulation showed more significant influence on the expression of the Cox7a2, Atp6v0d2 and Atp5j（P＜0.05）. Conclusions The pathway of oxidative phosphorylation may take part in the response of acupoint to moxibustion, Cox7a2, Atp6v0d2 and Atp5j may be the penitential target genes of oxidative phosphorylation pathway at ST36 after moxibustion. Key words Moxibustion, Oxidative phosphorylation, Cox7a2, Atp6v0d2, Atp5j, Gene Expression Profiles, Quantitative real-time PCR 目 录 中文摘要1 ABSTRACT 3 HYPERLINK \l _bookmark0 目 录5 HYPERLINK \l _bookmark1 缩略词表7 HYPERLINK \l _bookmark2 前言8 HYPERLINK \l _bookmark3 实验研究10 HYPERLINK \l _bookmark4 第一部分 艾灸后“足三里”穴位局部氧化磷酸化信号通路及其分子靶点的挖掘 10 HYPERLINK \l _bookmark5 1 实验材料 10