肠道寄生虫粪便病原学检查方法.docVIP

肠道寄生虫粪便病原学检查方法 　　[摘要] 在实验动物病原检测中，寄生虫检测是其中重要的分支，也是体现实验动物质量不可或缺的一个方面。寄生虫侵入宿主可在宿主体内产生多种有害作用包括夺取营养，机械性损伤，继发感染，毒性作用等，严重影响实验动物健康并降低实验动物的质量。许多寄生虫生活史中虫卵、卵囊、或幼虫随宿主的粪便排出体外，这些都可以通过粪便寄生虫检查，确定是否感染寄生虫以及感染的种类和程度。病原学检查是诊断寄生虫成虫及虫卵的重要的实验技术，采集粪便做粪便检查又是诊断实验动物消化道有无寄生虫感染比较常用的手段。实验动物粪检的病原学方法有多种。本文列举并介绍了一些目前比较常用的粪便寄生虫定性和定量的检查方法，旨在提高粪便寄生虫病原学检查的技术水平。 　　[关键词] 肠道寄生虫；粪便；病原学检查方法；实验动物质量 　　[中图分类号] R446 [文献标识码] B [文章编号] 2095-0616（2016）20-213-05 　　Pathogenic examination methods of Intestinal parasite stool 　　PAN Yan JIANG Zirui ZHOU Chao LI Caiyun 　　Shanghai Yinuosi Biological Technology Co.Ltd.，National Shanghai New Drug Safety Evaluation Research Center，Shanghai 201203，China 　　[Abstract] In the experimental animal pathogen detection，the parasite detection is one of the important branches，and it is also an indispensable aspect of the quality of experimental animals.Parasitic invasion of the host can produce a variety of harmful effects in the host body including the seizure of nutrients，mechanical damage，secondary infection，toxic effects，seriously affect the experimental animal health and reduce the quality of experimental animals.Many parasites in eggs and oocysts，or host larvae with the faeces excreted through feces，which can determine whether the parasite examination，parasite infection and infection type and degree.Etiological examination is an important diagnostic technique of parasite egg and adult feces collection，do stool examination is the diagnosis of digestive tract have no experimental animal parasitic infections more commonly used methods.There are many kinds of pathogenic methods in laboratory animal feces examination.This article lists and describes some of the more commonly used methods of qualitative and quantitative examination of fecal parasites，aimed at improving the technical level of fecal parasite pathogens. 　　[Key words] Intestinal parasites；Stools；Pathogenic examination method；Laboratory animal quality 　　根??现行的2001版中国国家标准实验动物寄生虫学检测方法，不同种属动物体内体外寄生虫有其适用的检测方法，这些方法是经过很