芩百清肺浓缩丸对肺炎支原体感染大鼠肺泡Ⅱ型上皮细胞修复作用研究.docVIP

芩百清肺浓缩丸对肺炎支原体感染大鼠肺泡Ⅱ型上皮细胞修复作用研究 　　[摘要]通过对转化生长因子β（TGFβ）和表面活性相关蛋白A（SPA）给药前后的表达变化及分布，探讨芩百清肺浓缩丸对肺炎支原体（MP）感染大鼠肺泡Ⅱ型上皮细胞的修复作用机制。取60只Wistar大鼠，雌雄各半，体重80～100 g，随机分为6组，每组10只，分别为空白组，模型组，阳性对照组，芩百清肺浓缩丸高、中、低剂量组，采用滴鼻法造模；给药10 d后收集大鼠肺泡灌洗液、血清、左肺及右肺组织中叶，采用免疫组化法检测肺组织中TGFβ及SPA的蛋白表达及分布；实时荧光定量PCR法测定肺组织中TGFβ，SPA mRNA的表达；酶联免疫法测定血清及肺泡灌洗液（BALF）中SPA的含量。通过数据分析可知，芩百清肺浓缩丸能够下调肺组织中TGFβ的表达，增加SPA的表达，其中TGFβ主要分布于肺间质，SPA主要分布于AECⅡ及部分肺泡巨噬细胞内；同时，能够降低血清中SPA含量，增加BALF中SPA含量。通过以上结果可知，芩百清肺浓缩丸通过降低TGFβ的表达抑制AECⅡ发生上皮间质转化，并增加SPA的表达，恢复肺的正常形态与功能；同时，对MP引起的肺组织毛细血管损伤具有一定的修复作用。 　　[关键词]肺炎支原体；芩百清肺浓缩丸；肺泡Ⅱ型上皮细胞；转化生长因子β；表面活性相关蛋白A 　　[Abstract]To discuss the repair mechanism of Qinbai Qingfei concentrated pellets to AECⅡ of rats infected by mycoplasma through the observation of the changes and distribution of TGFβ and SPA in lungs， totally 60 Wistar rats that weighing 80100 g were collected， with male and female in half The rats were divided into six groups randomly， with 10 rats each group， namely blank group， model group， positive group and Qinbai Qingfei concentrated pellets high， middle and low dose groups Rats were infected through nasal intubation drip of MP After 10 days of administration， serum and bronchoalveolar lavage fluid （BALF） were collected to detect the concentration of surface activity related protein A （SPA） by ELISA， left pulmonary tissues of rats were collected to observe the expression and distribution of transforming growth factorbeta （TGFβ） and SPA by immunohistochemistry， and right pulmonary tissues were taken to detect TGFβ and SPA mRNA expression level by realtime quantitative PCR （RTPCR） Qinbai Qingfei concentrated pellets can reduce the expression of TGFβ and increase the expression of SPA in the lung tissues of rats infected by mycoplasma Specifically， TGFβ was mainly distributed among the lung interstitium， while SPAs were mainly distributed in AECII and parts of alveolar macrophage The level of SPA was reduced in serum and increased in BALF in rats in Qinbai Qingfei concentrated pellets groups