血管内皮生长因子受体在胃癌浸润和转移中作用探讨.docVIP

血管内皮生长因子受体在胃癌浸润和转移中作用探讨 　　[摘要] 目的 探讨血管内皮生长因子受体（KDR）在胃癌中的表达及其临床意义。方法 收集60例手术切除的胃癌标本，同时取距离肿瘤组织5cm以上的正常组织，采用免疫组织化学SP法检测癌组织及癌旁组织中KDR的表达。结果 60例患者中胃癌组织KDR的阳性率为55%，癌旁组织中为18.3%，KDR在胃癌组织内的阳性表达率明显高于癌旁组织，差异有统计学意义（P＜0.01）；KDR的阳性表达率随着胃癌组织的TNM分期Ⅰ～Ⅳ期进展不断增高，具有统计学意义（P＜0.05）。结论 KDR在胃癌组织中存在高表达，且其与胃癌的TNM分期密切相关，通过检测KDR可能会对我们评估胃癌患者的预后情况提供一定的帮助。 　　[关键词] 胃癌；KDR；免疫组化 　　[中图分类号] R735.2[文献标识码] B [文章编号] 1673-9701（2011）27-98-02 　　 　　The Role of Vascular Endothelial Growth Factor Receptor（KDR）in Invasion and Metastasis of Gastric Cancer 　　YANG YingqiangSONG Zhenya 　　Department of Gastroenterology, Luqiao Hospital of Taizhou City in Zhejiang Province, Taizhou 318050, China 　　 　　[Abstract] Objective To study the expression of vascular endothelial growth factor receptor KDR in gastric cancer and its clinical value. Methods Collected 60 specimens from gastric cancer patients. The expression of KDR in gastric cancer tissues and their adjacent normal tissues were detected with SP immunohistochemistry. Results The positive rate of KDR expression in gastric cancer tissue was higher than that in normal tissue（55.0% vs 18.3%）. There was significant difference in the expression of KDR between the gastric cancer tissue and their adjacent normal tissue（P＜0.01）. Besides, the expression rate of KDR in gastric cancer tissue was correlated with gastric cancer TNM stages（P＜0.05）. Conclusion KDR is highly expressed in gastric cancer tissues, and it is closely related to the TNM staging of gastric cancer. To detect the expression of KDR in gastric cancer tissues may be helpful to evaluate the prognosis of patients with gastric cancer. 　　[Key words] Gastric cancer；KDR；Immunohistochemistry 　　 　　胃癌作为消化系统常见的恶性肿瘤之一，其死亡率一直位居所有恶性肿瘤之首。研究表明血管的形成是与肿瘤的生长转移密切相关的条件，而大量抗血管生长因子和促血管生长因子的共存，在调节血管形成过程中起重要作用，其中血管内皮生长因子（VEGF）通过与细胞膜上相应的受体（VEGFR）结合在调节血管的形成过程中起主导作用。人血管内皮生长因子受体-2基因（KDR）多分布在血管内皮细胞上，但有研究发现肿瘤细胞KDR表达更强于内皮细胞，其与肿瘤新生血管形成的关系受到越来越多学者的关注。本实验对不同胃癌组织的VEGF受体KDR用免疫组化法进行检测，并将其和正常的组织作为对照，以探讨血管内皮生长因子受体（KDR）与胃癌浸润和转移的关系。 　　1材料与