比目鱼皮抗氧化肽的制备及分离纯化-化工过程机械专业论文.docxVIP

as follows: pH 6.62, temperature 48.2 oC and E/S 2776 U/g. Under the optimal hydrolysis conditions, the relative error between the predicted value and experimental value of DPPH scavenging activity was 1.04%. The molecular weight of collagen peptides was distributed between 900~1600 Da. And amino acids associated with antioxidant activity were rich in collagen peptides. A novel antioxidant peptide was isolated from antioxidant peptides by Sephade x G-15 gel filtration chromatography, Sephade x G-10 gel filtration chromatography and RP-HPLC. The molecular weight measured by MS was 1025.9 Da, may be octapeptide. The parameters of the preparation of collagen peptides were amplified. Results showed that peptides yield was 60.82% and purity was 75.05%. The molecular weight of collagen peptides manufactured by amplified experiment was distributed between 500~1600 Da. The economic benefit of annual output of 10 tons collagen peptides was analyzed, showing that annual sales income 5 million yuan was obtained and net profit was 3.224 million yuan. Two new for ms of collagen peptide products were developed：Oral liquid containing collagen and collagen effervescent tablet were produced. KEY WO RDS：Flatfish skin, Collagen peptides, Antioxidant, P urification 目 录 HYPERLINK \l _bookmark0 第一章 文献综述1 HYPERLINK \l _bookmark1 1.1 多肽1 HYPERLINK \l _bookmark2 1.1.1 多肽的吸收机制1 HYPERLINK \l _bookmark3 1.1.2 几种典型的生物活性肽2 HYPERLINK \l _bookmark4 1.2 生物活性肽的制备方法5 HYPERLINK \l _bookmark5 1.3 抗氧化活性肽的国内外研究进展7 HYPERLINK \l _bookmark6 1.3.1 抗氧化活性的评价标准7 HYPERLINK \l _bookmark7 1.3.2 酶解水产蛋白制备抗氧化肽的研究进展8 HYPERLINK \l _bookmark8 1.3.3 抗氧化肽的分离纯化13 HYPERLINK \l _bookmark9 1.4 课题背景、意义及研究内容16 HYPERLINK \l _bookmark10 1.4.1 比目鱼皮资源利用现状16 HYPERLINK \l _bookmark11 1.4.2 国内多肽生产工艺的现状16 HYPERLINK \l _bookmark12 1.4.3 本论文主要研究内容17 HYPERLINK \l _bookmark13 第二章 比目鱼皮预处理工艺的研究18 HYPERLINK \l _bookmark14 2.1 材料和仪器18 HYPERLINK \l _bookmark15 2.1.1 材料及试剂18 HYPERLINK \l _bookmark16 2.1.2 主要