补阳还五汤对局灶性脑缺血大鼠神经功能及BrdU影响.docVIP

补阳还五汤对局灶性脑缺血大鼠神经功能及BrdU影响 　　【摘要】目的 探讨补阳还五汤对局灶性脑缺血大鼠神经功能及BrdU的影响。方法 采用大脑中动脉线栓法建立右侧局灶性脑缺血大鼠模型，将动物随机分为正常组、假手术组、模型组和补阳还五汤组，腹腔注射Brdu，分别于1d、7d、14d和28d处死动物，采用免疫组织化学法观察Brdu表达情况。结果 正常脑内存在一定的BrdU阳性细胞，脑缺血后表达迅速增加，7天达高峰，然后逐渐下降；补阳还五汤可显著增强BrdU的表达，在第7d、14d时明显多于其它各组（P0.05），用药7、14d，补阳还五汤组降低神经功能积分作用与其它用药组比较差异有统计学意义（P0.05）。结论 补阳还五汤可促进局灶性脑缺血大鼠缺血区BrdU的表达及神经功能恢复。 　　【关键词】补阳还五汤 脑缺血 5-溴脱氧尿嘧啶核苷 大鼠 　　中图分类号：R743.31文献标识码：A文章编号：1005-0515（2010）12-034-03 　　Effect of Buyang Huanwu Decoction on BrdU in Rats after Focal Cerebral Ischemia 　　【Abstract】Objective To explore the effect of Buyang Huanwu Decoction(BYHWD) on BrdU in rats after focal cerebral ischemia．Methods The model of focal cerebral ischemia in rats was reproduced by middle cerebral artery occlusion．Rats were randomly divided into following groups including navie,shame-operated,ischemia and Buyang Huanwu Decoction(BYHWD) groups．Animals were received BrdU-labeled and killed at 1,7,14 and 28 day point after operation.BrdU was measured by immunohistochemical staining．Result There was a certain BrdU positive cells in normal brain, after cerebral ischemia BrdU positive cells were increased and reached the peak at 7 day．BYHWD can significantly increase the expression of BrdU．The increase was statistically significant at 7 and 14 day point after ischemia（P0.05）．Compared to the other groups，there was statistically significant on BYHWD for decreasing the number of nerve function at 7 and 14 day points（P0.05）．Conclusion BYHWD may promote the expression of BrdU in rats after focal cerebral ischemia．Key words：Buyanghuanwu decoction；Cerebral ischemia；BrdU；Rat 　　 　　缺血性脑血管疾病日益危害着人类的健康，是高发且高危的脑血管疾病之一，尽快恢复缺血区的血供对神经功能的恢复至关重要。补阳还五汤是中医治疗缺血性脑疾病的有效方剂，能显著增强脑缺血后VEGF及其受体Flk1的表达[1]，本研究通过免疫组化方法观察脑缺血后BrdU的表达，探讨补阳还五汤是否可以促进脑缺血大鼠缺血区BrdU的表达。 　　1 材料与方法 　　1.1材料 　　1.1.1 动物及药物 清洁级SD大鼠，体重250～300g，雌雄各半，购于北京实验动物中心。补阳还五汤处方来源于清?王清任《医林改错》，按原方：黄芪120g、赤芍10g、川芎10g、当归尾10g、地龙（研末）10g、红花10g、桃仁10g，中药饮片购自湖南省中医院，鉴定合格，按传统方法制备补阳还五汤汤剂并冷藏备用。 　　1.1.2 试剂及仪器 BrdU单克隆抗体购自美国Chemico公司；SABC