电针耳穴对d半乳糖致豚鼠老年性聋听觉中枢mda表达的影响-耳鼻咽喉科学专业论文.docxVIP

 PAGE 10 模型组豚鼠出现老化症状:毛色逐渐枯楠，头顶部，背部，腹部掉毛 严章，进食减少，耳廓反射灵敏度下降，体重与对照组相比有所降低 (P0.05)，对照组外观表现如实验前， D帽gal+ 咆针组和老年豚鼠组 外观表现介于 D-gal 模型组和对照组之间， D唱al+电针组体重与 D也al 棋型组相比无明显变化 (P0.05)0 (2) ABR 测定结果:对照组、 D-gal 模型组、D鸣al吨针组和老年组的 ABRIII 波潜伏期的值分别为: 0.89 士0.88 、106.士0.14、0.90 士 0.10、 1.00 士0.10 ms ，与对照组比 较， D-gal 模型组111波潜伏期延长 (P0.05); 与 D-gal 模型比较， D也al+ 电针组III波潜伏期缩短 (P0.05)0 (3) D-gal 模型组豚鼠听 皮层、下丘和耳蜗核 MDA 含最明显高于空白对照组 (P0.05); 老 年组豚鼠昕皮层、下lí.和耳蜗核 MDA 含最显著南于空白对照细 (P 0.05); D噶al+电针组与 D-gal 模型组相比，三个部位的如 IDA 含量 显著降低 (P0.05); D唱al 模型组与老年组相比，三个部位的 MDA 含量无明显差异 (P0.05)。结论:(1) D-gal 300mg/kg.day 颈部皮 下注射 6 周可建立 AHL 豚鼠模型。 (2) D唱al 可以导致豚鼠昕觉中 枢的老化。 (3) 昕觉中枢的老化可能与 MDA 含量升高所导致的脂 质过氧化有关。 (4) 针刺昕宫和黯风两个穴位可在…定程度上抑制 D翩 gal 所致的豚鼠昕觉中枢的老化过程。 关键词: 老年性聋;电针:昕皮层:下丘:耳蜗核:内工醒 Effects of EIectroacupuncture at Tinggong and Yifeng on MDA expression in auditory center of guinea pigs with age幽 reIated hearing Ioss by D-gaIactose Abstract Objective: Agφrelated hearing loss (AHL) is a degenerative disease， which severely affects the physical and mental health of the old people. Because of the long course and bad curative effect of AI伍， the ear1y intervention to the AHL research is necessary. Acupuncture has the early characteristic of early intervention ，and should play an important role in the prevention and treatment of AHL.The purpose of the study is to establish a model of guinea pigs with AHL by subcutaneous injection of D噶alactose，and investigate the effects of MDA on the mechanism of AHL and the changes in the expression of MDA in auditory cortex， inferior colliculus and cochlear nucleus of guinea pigs with AHL a位er electroacupunc阳re at Tinggong and Yifeng. Methods: There were 4 groups. 30 adult guinea pigs were randomly divided into 3 groups including the D-galactose group (DG， n斗的， the D-galactose and electroacupuncture group (DEG，n叶的， and control group (CG，n=10). ηle fourth group was the elderly group (n=10). Guinea pigs of the DG and DEG groups were su