第10章 DNA的复制.ppt

Ⅱ The extension of DNA replication Section 4 Reverse transcription and others  Section 5DNA damage and repair transvertion Transversions are changes from a pyrimidine to either of the two purines, and or the changes of a purine into either of the two pyrimidines. C G C A T G T A transversion C G C G G 1 C C G C G C G A 2 C T A T A C A G 3 C T A T A C A A 4 C C G A C A T G C G T A T C T C 5 C G C A T C T T 6 C T T G C C C C 7 C G C G C C C T 8 C A T HbA ? HbS ? gene peptide gene peptide Glu Val CTC CAC GAG GTG 17T?A, 6Glu?Val point mutation C A C G T A T G A C A T G C G T transversions transitions C T T C A G G A deleted mutation inserted mutation inversed mutation 5’ 5’ 3’ 3’ 5’ 5’ 3’ 3’ OH OH OH OH P P P P 5’ 5’ 3’ 3’ OH P P 5’ 5’ 3’ 3’ OH OH OH P P rearrangement ? ?1 ?2 ? ? Hb ? genes family in 11 chromosome ?1 ?2 ? ? ?1 ?2 ? ? ?1 ?2 ? ? ?1 ?2 ? ? ? ? Hb anti-Lepore Hb Lepore two gene types of Med anaemia repair of DNA damaged 1 photoreactivation repair 2 excision repair 3 recombination repair 4 SOS repair Photoreactivation repair O O O O P H N N R H N N R CH3 CH3 UV photolyase 300-600nm O O O R P H N N R CH3 O H N CH3 N excision repair affect factor specific endo- nucleases 3’ 5’ ligase 5’ 3’ 5’ 3’ pol 1 Uvr A Uvr B Uvr C recombination repair pol I ligase RecA SOS repair ? The DNA is damaged gravely and extensively. ? The replication of DNA had been stoped. ? The SOS repair is a emergency step. ? The SOS repair is a complicated network of regulation. ? The rate of DNA mutation is still very high. ? The SOS repair is called error prone repair. ? ? ? ? ? ? ? 1 13 17 29 32 44 55 66 166 174 201 209 237 245 TGTGGATTA---TTATACACA------TTTGGATAA---TTATCCACA GATCTNTTTATTT---GATCTNTTNTATT---GATCTCT