银翘散对呼吸道黏膜免疫功能低下合并甲型流感病毒感染模型小鼠影响.docVIP

银翘散对呼吸道黏膜免疫功能低下合并甲型流感病毒感染模型小鼠影响 　　摘要：目的 观察银翘散对呼吸道黏膜免疫功能低下合并流感病毒感染模型小鼠的影响，并探讨其作用机制。方法 复制寒冷刺激致小鼠上呼吸道黏膜免疫功能低下模型，采用甲型流感病毒鼠肺适应株A/PR/8/34 （H1N1）通过鼻腔接种方式感染模型小鼠，将小鼠随机分为正常组、模型组、阳性药（利巴韦林）组及银翘散组，各给药组给予相应药物灌胃，观察各组小鼠死亡率、生命延长率、平均存活时间及肺指数。结果 与模型组比较，阳性药组、银翘散组小鼠死亡率明显降低（P0.05，P0.01），存活时间延长；阳性药组、银翘散组小鼠肺指数明显降低（P0.05，P0.01），肺指数抑制率分别为35.5%、24.6%。结论 银翘散通过上调模型小鼠上呼吸道局部黏膜免疫功能，达到防治上呼吸道感染的作用。 　　关键词：银翘散；流感；黏膜免疫；小鼠 　　DOI：10.3969/j.issn.1005-5304.2015.08.019 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）08-0070-03 　　Effects of Yinqiao Powder on Mouse Models with Upper Respiratory Trace Mucosal Immunity Dysfunction Infected with Influenza Virus A LIU Li-song， YIN Hong， WANG Wei-li， YAN Han-wen， LIN Qing， Lei Na （Yunnan University of Traditional Chinese Medicine， Kunming 650500， China） 　　Abstract：Objective To observe the effect of Yinqiao Powder on the mouse models with upper respiratory trace mucosal immunity dysfunction infected with influenza virus A， and explore mechanism of action. Methods The mouse models of upper respiratory trace mucosal immunity dysfunction induced by cold stimulation with the influenza A/PR/8/34 （H1N1） virus through the nasal cavity were established. The mice were randomly divided into normal group， model group， positive medicine （Ribavirin） group， and Yinqiao Powder group. All administration groups received gavage with relevant medicine， and then mortality， the life prolonging rate， average survival time and the lung index of each group were observed. Results Compared with the model group， the mortalities in positive medicine group and Yinqiao Powder group decreased significantly （P0.05， P0.01）， with longer survival time. The lung indexes in positive medicine group and Yinqiao Powder group decreased significantly （P0.05， P0.01）， and the inhibition ratios of lung index were 35.5% and 24.6%， respectively. Conclusion Yinqiao Powder can realize the protective effects on upper respiratory infection through upregulating the mucosal immunity of the upper respiratory tract o