醒鼻温敏凝胶剂对变应性鼻炎大鼠血清白细胞介素―4转化生长因子―β1和鼻黏膜嗜酸性粒细胞影响.docVIP

醒鼻温敏凝胶剂对变应性鼻炎大鼠血清白细胞介素―4转化生长因子―β1和鼻黏膜嗜酸性粒细胞影响 　　摘要：目的 观察醒鼻温敏凝胶剂对变应性鼻炎（AR）大鼠血清白细胞介素-4（IL-4）、转化生长因子-β1（TGF-β1）及鼻黏膜嗜酸性粒细胞（EOS）计数的影响，探讨其作用机制。方法 以卵清白蛋白加氢氧化铝腹腔注射基础致敏，局部以2%卵清白蛋白激发成功复制AR模型。实验大鼠随机分为正常组、模型组、阳性对照组和醒鼻低、中、高剂量组，各给药组给予相应药物干预。比较各组大鼠症状评分，检测血清IL-4、TGF-β1含量和鼻黏膜EOS计数。结果 与正常组比较，模型组大鼠血清IL-4、TGF-β1含量明显升高（P0.01），鼻黏膜组织EOS计数明显增加（P0.05）；与模型组比较，醒鼻中剂量组和阳性对照组血清IL-4、TGF-β1含量明显降低（P0.01），鼻黏膜?M织EOS计数明显减少（P0.05）。结论 醒鼻温敏凝胶剂可能通过下调IL-4、TGF-β1等炎症因子水平及减少鼻黏膜EOS浸润而发挥治疗作用。 　　关键词：醒鼻温敏凝胶剂；白细胞介素-4；转化生长因子-β1；嗜酸性粒细胞计数；大鼠 　　DOI：10.3969/j.issn.1005-5304.2018.02.011 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）02-0047-04 　　Effects of Xingbi Wenmin Gel on Serum Levels of IL-4， TGF-β1 　　and Nasal Mucosa Eosinophils in Allergic Rhinitis Rats 　　QIU Cai-xia1， ZHENG Jian2， LIN Dong-hong1， AI Si1， WU Bo3， 　　ZHUANG Xiang-li2， WENG Ke-han1， LI Xu-wei1 　　1. People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine， Fuzhou 350004， China； 　　2. Fujian University of Traditional Chinese Medicine， Fuzhou 350122， China； 　　3. The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine， Fuzhou 350001， China 　　Abstract： Objective To observe the effects of Xingbi Wenmin Gel on serum levels of IL-4， TGF-β1 and nasal mucosa eosinophils （EOS） in allergic rhinitis （AR） rats； To discuss its mechanism of action. Methods Intraperitoneal injection of egg albumin and aluminum hydroxide based sensitized to ovalbumin challenge 2% local AR model was established successfully. The experimental rats were randomly divided into normal group， model group， positive control group， and Xingbi Gel low-， medium-， and high-dosage groups. Each medication group was given relevant medicine for intervention. The rat symptom scores in each group were compared， and serum IL-4， TGF-β1， and nasal mucosa EOS counts were detected. Results Compared with the normal group， the serum levels of IL-4 and TGF-β1 in model group increased significantly （P0.01）， and the nasal mucosa EOS counts increased s