鲤细菌性败血症免疫研究.docVIP

鲤细菌性败血症免疫研究 　　摘要：选取分离于鲤细菌性败血症病例的嗜水气单胞菌强毒力菌株进行复壮，于菌悬液中加入0.2% 甲醛于28 ℃ 灭活12 h制成灭活疫苗，对鲤进行免疫接种后，通过检测抗体, 以确定鱼体对疫苗的应答强度。分别取注射免疫后第21，28，35，42天的血清做凝集试验，并于首免6周后做人工感染保护试验。结果抗体最高效价达到 212，而对照组为0。受免组攻毒免疫保护率达90%，表明鱼体对嗜水气单胞菌灭活疫苗可以产生免疫应答, 对鲤有一定的免疫保护作用。 　　关键词：细菌性败血症；鲤；免疫 　　中图分类号：S941.42+9文献标识码：ADOI编码：10.3969/j.issn.1006-6500.2011.01.027 　　 　　Immunological Study of Bacterial Septicemia in Carp 　　ZHANG Yu-fen1,2, ZHANG Wen-li1,LI Xu-kun1,YANG Sen1, ZHANG Xiu-jun1 　　(1.Fengrun Center of Disease Control and Prevention ,Tangshan, Hebei 064000, China; 2.Department of Life Science, North China Coal Medical College, Tangshan, Hebei 063000, China) 　　Abstract：Bacterial liquid were obtained after rejuvenating from selected strong virulence strains ofAeromonas hydrophila isolated from bacterial septicemia of carp, which was made into vaccine by inactivating it with 0.2% formaldehyde at 28 ℃ for 12 hours. The inactivated vaccine was inculated carps through abdominal cavity. The intensity of response in fish to stimulation of vaccine was determined through examine immune antibody. Examination of the agglutinating antibody titers on the 21th , 28th , 35th , 42th days after vaccination showed that the highest titer of the immunized carp was 212, while that of the control was 0, antibody levels were significatly different between immunized group and control group(P0.05). The relative percent survival of the immuned group was 90% by experiments about protection to artificial infections by immuned six weeks later. The resuls showed that fish which was immuned could create 9 times immune antibody response to immune of inactivated vaccine of Aeromonas hydrophila, had a certain protective effective effect on Carp. 　　Key words: bacterial septicemia；carp；immune 　　 　　细菌性败血病(bacterial septicemia)20世纪70年代始发于日本，80年代在中国台湾流行，1989 年传入中国大陆[1]。自 1989 年以来，各种淡水鱼类细菌性败血病频繁发生[2-6]。嗜水气单胞菌为本病的主要病原菌，广泛分布于池塘、溪、涧、江、河、湖泊和邻泊河口等淡水环境，因此感染范围也十分广泛。目前，越来越多的水产学专家研究疫苗用于防治鱼类细菌性败血症。为探讨疫苗的防治效果，笔者采用自然患细菌性败血症的鲤 (Cyprinus Carpio