鹿特异性复合麻醉剂对大鼠大脑皮层Na―K―ATP酶活性影响.docVIP

鹿特异性复合麻醉剂对大鼠大脑皮层Na―K―ATP酶活性影响 　　摘要：通过测定鹿特异性复合麻醉剂麻醉下大鼠大脑皮层Na+-K+-ATP酶活性的变化，探讨了其麻醉与大鼠大脑皮层Na+-K+-ATP酶的关系。将32只纯种SD大鼠随机分成对照组、诱导组、麻醉组和催醒组，对照组大鼠按30 mL/kg体重腹腔注射生理盐水，其余各组按30 mg/kg体重腹腔注射鹿特异性复合麻醉剂，于各时间点冰上采集各组大鼠大脑皮层，采用比色法测定其Na+-K+-ATP酶活性。结果显示，药物作用后大鼠大脑皮层Na+-K+-ATP酶活性显著低于对照组（P0.05或P0.01）。该结果提示大鼠大脑皮层Na+-K+-ATP酶活性变化与鹿特异性复合麻醉剂的麻醉作用具有相关性。 　　关键词：鹿特异性复合麻醉剂；大鼠；大脑皮层；Na+-K+-ATP酶 　　中图分类号：S859.79+1 文献标识码：A 文章编号：0439-8114（2016）04-0964-02 　　DOI：10.14088/j.cnki.issn0439-8114.2016.04.037 　　Effects of Specificity Anesthetic for Deer on Na+-K+-ATPase Activity 　　in Cerebral Cortex of Rats 　　FENG Xue， FU Lian-jun， LIN Bo， CHANG Nai-yuan， LI Jing-cheng， LI Wen-xuan， YIN Bai-shuang 　　（Department of Animal Medicine， Jilin Agricultural Science and Technology， Jilin 132101，Jilin，China） 　　Abstract： This experiment was aimed to determine the activity of Na+-K+-ATPase in rats cerebral cortex region and investigate the relationship between specificity anesthetic for deer and Na+-K+-ATPase in cerebral cortex. 32 SD rats were randomly divided into control group， induction group， anesthesia group and the wake group. Rats of the control were injected with saline（30 mL/kg），rats of the other group were injected with drug （30 mg/kg）. The samples of cerebral cortex in different period were separated and the activities of Na+-K+-ATPase were determined by using colorimetry methods. The results showed that the activities of Na+-K+-ATPase in cerebral cortex after administration were decreased obviously compared with that in the control group（P0.05 or P0.01）. It indicated that the changes of the activity of Na+-K+-ATPase in the cerebral cortex were correlated with the specificity anesthetic for deer. 　　Key words： specificity anesthetic for deer； rats； cerebral cortex； Na+-K+-ATPase 　　在哺乳动物脑组织中Na+-K+-ATP酶含量丰富，该酶在维持神经细胞的兴奋性、传导性及调节神经递质代谢等方面发挥着重要作用[1]。动物在麻醉过程中脑组织神经细胞处于抑制状态，神经细胞的转导作用受阻。过去的研究表明，多种麻醉药物均可引起跨膜信号转导系统中Na+-K+-ATP酶活性降低，从而调节细胞内Ca2+浓度，控制神经递质的释放，抑制神经细胞之间及神经细胞与肌肉或腺体细胞之间的兴奋传递，产生麻醉现象[2]。鹿特异性复合麻醉剂是吉林农业科技学院动物外科