黄芪参附对低位中枢神经肌接头和骨骼肌疲劳影响.docVIP

黄芪参附对低位中枢神经肌接头和骨骼肌疲劳影响 　　【摘要】目的：观察黄芪注射液和参附注射液对低位中枢、神经-肌接头和骨骼肌疲劳的影响。方法:记录蟾蜍坐骨神经干动作电位和腓肠肌收缩曲线，测定低位运动中枢、神经-肌接头和骨骼肌出现疲劳的时间。结果：与对照组比较，黄芪组和参附组低位中枢、神经-肌接头和骨骼肌疲劳的时间均明显延长(P0.05)；而与黄芪组比较，参附组神经-肌接头和骨骼肌疲劳时间延长，差异具有显著性(P0.05)。结论：黄芪注射液和参附注射液具有抗低位中枢、神经-肌接头和骨骼肌疲劳的作用。 　　【关键词】黄芪；参附；神经-肌接头；骨骼肌；蟾蜍 　　文章编号：1009-5519（2007）16-2375-02 中图分类号：R33 文献标识码：A 　　Effects of astragalus membranacens and shenfu on fatigue in lower nerve centre neuromuscular junction and skeletal muscle 　　ZOU Hai-feng，CHEN Yan，LIN Yan 　　(Hospital of Luzhou Armed Police Forces，Luzhou 646000，China) 　　【Abstract】Objective：To study the effects of astragalus membranacens injection and shenfu injection on fatigue in lower nerve center， neuromuscular junction and skeletal muscle.Methods：By recording the action potential of sciatic nerve trunk and the contractive curve of gastrocnemius muscle，and measuring the time of fatigue in lower nerve centre，neuromuscular junction and skeletal muscle.Results:In both the astragalus membranacens group and the shenfu group，the time of fatigue in nerve center，neuromuscular junction and muscle was increased，and the differences were significant compared with the control group(P0.05). The time of fatigue of the shenfu group in neuromuscular junction and skeletal muscle was longer than that of the astragalus membranacens group，and the differences were significant(P0.05). Conclusion: Both the astragalus membranacens injection and the shenfu injection could apparently resist the fatigue in nerve center，neuromuscular junction and skeletal muscle. 　　【Key words】Astragalus membranacens；Shenfu；Neuromuscular junction；Skeletal muscle；Toad 　　 　　已有研究表明，黄芪注射液和参附注射液能提高机体抗氧化、抗骨骼肌疲劳和抗缺氧的能力[1，2]，但两种制剂对同一动物的运动中枢、神经-肌接头和骨骼肌有无抗疲劳的作用，以及两者抗疲劳作用的比较，尚未见报道。本试验通过观察蟾蜍坐骨神经干动作电位和腓肠肌收缩，探讨黄芪和参附对低位中枢、神经-肌接头和骨骼肌疲劳的影响，比较两者的抗疲劳作用，并分析其可能机制。 　　 　　1 材料和方法 　　 　　1.1 动物分组：30只100~150 g的成年蟾蜍，雌雄不拘（泸州医学院动物科提供），随机均分为对照组、黄芪组（黄芪剂量0.8 g/kg，相当于成人剂量的20倍）和参附组（参附剂量4 ml/kg，相当于成人剂量的20倍）3个组。 　　1.2 药品：黄芪注射液（正大青春宝药业有限公司，批号0311192）每支10 ml，