黄芪多糖对中波紫外线辐射皮肤角质形成细胞损伤影响.docVIP

黄芪多糖对中波紫外线辐射皮肤角质形成细胞损伤影响 　　摘要：目的 观察黄芪多糖对中波紫外线（UVB）辐射损伤皮肤角质形成细胞（HaCaT细胞）的影响，初步探讨其作用机制。方法 采用UVB辐射HaCaT细胞进行造模。分为空白对照组、模型组、阳性对照组及黄芪多糖低、中、高剂量组，各药物组给予相应药物干预。MTT法测定细胞活力；生化比色法检测谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、丙二醛（MDA）含量；ELISA检测肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）含量。结果 与空白对照组比较，模型组SOD、CAT、GSH-Px含量降低，MDA、TNF-α、IL-6含量升高（P0.05）；与模型组比较，各药物组SOD、GSH-Px、CAT含量升高，MDA、TNF-α、IL-6含量降低（P0.01）。结论 黄芪多糖可在一定程度上减轻UVB对HaCaT细胞氧化应激性损伤。 　　关键词：黄芪多糖；中波紫外线；皮肤角质形成细胞；保护作用 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）05-0044-03 　　Effects of Astragalus Polysaccharides on Damage of Keratinocytes in UVB Radiation Skin LI Yang， An Fang-yu， Ming Hai-xia， LIU Yong-qi （Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research， Gansu University of Chinese Medicine， Lanzhou 730000， China） 　　Abstract： Objective To investigate the effects of Astragalus polysaccharide （APS） on damage of keratinocytes in ultraviolet B （UVB） radiation skin in HaCaT cells； To preliminarily explore its mechanism of action. Methods HaCaT cells were treated by UVB irradiated for modeling. Blank control group， model group， UVB irradiation group and low-， medium- and high-dose APS interventional groups were set up. Each medication group was given relevant medicine. MTT assay was used to determine the cell activity； GSH-Px， CAT， SOD activity and MDA content were detected by biochemical colorimetric method； contents of TNF-α and IL-6 were detected by ELISA. Results Compared with the control group， the contents of SOD， CAT and GSH-Px in the model group decreased significantly， while the contents of MDA， TNF-α and IL-6 increased significantly （P0.05）； compared with the mode group， the contents of SOD， CAT and GSH-Px in the medication groups increased significantly， while the contents of MDA， TNF-α and IL-6 were reduced significantly （P0.01）. Conclusion APS can reduce the oxidative stress injury of UVB to HaCaT cells to some extent. 　　Key words：