杜仲抗骨质疏松药效物质基础及机制研究-药剂学专业论文.docx

RT-PCR 方法对成骨细胞 TRACP 因子考察发现，京尼平苷酸能浓度依赖性的抑 制 TRACP 因子的活性。结论：杜仲 40%乙醇组分是促成骨细胞增殖及分化成 熟活性组分，该组分中的化合物紫云英苷、黄芩苷、京尼平苷酸和咖啡酸是杜 仲抗骨质疏松的活性成分。京尼平苷酸、紫云英苷、黄芩苷、咖啡酸具有显著 性的提高成骨细胞分化成熟的能力并且京尼平苷酸还能抑制成骨细胞转化为破 骨细胞，维持成骨细胞与破骨细胞的平衡。同时京尼平苷酸、紫云英苷、黄芩 苷、咖啡酸能上调 OPG/RANKL 系统改善骨重建；紫云英苷、黄芩苷加强钙离 子与骨组织的结合能力辅助改善骨重建以到达改善骨质疏松症的目的。综上所 述，本研究初步阐明了杜仲药材抗骨质疏松的药效物质基础，并对其抗骨质疏 松的机制进行了初步研究，为杜仲药材的开发应用提供了实验依据。 关键词：杜仲；骨质疏松；药效物质基础；机制研究 V Studies of pharmacodynamic material basis of Eucommia ulmoides Oliv Major: Pharmacy Master Candidate: Lan Bo Sipervisors: Li Yong-Jun Lan Yan-Yu [Abstract] Objective: the application of modern separation technology of Eucommia ulmoides were isolated and purified by the research methods and mechanism of pharmacology research Du Zhongkang osteoporosis. Methods: the chemical composition separation of Eucommia ulmoides by drilling resin adsorption method, use UPLC maps; Using the research methods of pharmacology, in vitro culture of MC3T3-E1 subclone 14 into bone cells and SAO-2 human osteoblasts, and study the effects of Eucommia ulmoides components of MC3T3-E1 subclone 14 into bone cells and SAO-2 human osteoblast cell survival rate, alkaline phosphatase activity, osteoprotegerin activity. Through the research of pharmacology, chemical components of Eucommia ulmoides in established HPLC (UPLC) map based on it. With the help of electrospray ionization - by ultra performance liquid chromatography tandem mass spectrometry (UPLC-ESI-MS/MS) combined with technology of Eucommia contributed to the proliferation of bone cell effective component research. Investigation of Eucommia compound on MC3T3-E1 subclone 14 into the survival rate of bone cells by pharmacology effect method, and application of real time fluorescent quantitative PCR and Western blot (Western blot) technology research at the molecular level of Eucommia compound on MC3T3-E1 subclone 14 in osteoblasts of OPG, RANKL, RUNX-2, expression of Osterix and TRACP gene and protein the change of the ratio of OPG/RANKL, investigation. A