独一味有效部位的筛选及其制剂研究-中药药剂学(中药炮制)专业论文.docxVIP

摘 要 独一味 Lamioplomis rotata (Benth.) Kudo 是唇形科独一味属植 物，生长于海拔 4000m 左右的高山草甸、河滩等处，主产于西藏、 青海、云南、四川、甘肃等省区，具有止血、镇痛消肿、活血化瘀、 抗菌消炎、增强免疫力等功效。 国内外学者研究表明，独一味主要含有黄酮、环烯醚萜苷、苯乙 醇苷等成分，但反映独一味临床功效与药理作用的有效成分尚不明 确。我们从四川石渠县采集独一味药材，将其醇提取物分为石油醚、 乙酸乙酯、正丁醇、水 4 个部位，分别采用小鼠醋酸扭体法和断尾止 血法对各部位进行了初步药效学试验，结果表明，独一味的正丁醇部 位能显著减少小鼠扭体反应次数以及缩短出血时间。对正丁醇提取部 分大孔树脂水洗部分、30%、90%乙醇洗脱部位，继续采用小鼠醋酸 扭体法和断尾止血法筛选，其中 30%乙醇洗脱部位对小鼠具有明显的 镇痛、止血作用。 在有效部位的研究基础上，制备得到了以有效部位为主的独一味 中间体。为了提高独一味制剂的生物利用度，减少服用量，我们以中 间体为原料制备了独一味滴丸，并且建立了质量标准控制体系。 关键词：独一味 有效部位 制剂 ABSTRACT Lamioplomis rotata (Benth) Kudo, a plant of lamioplomis genus (labiatae family),widely grows in alpine meadow and flood land at altitude 4000m. It distributes in Tibet, Mongolia and Naxi people to promote blood circulation, remove blood stasis, subdue swelling and alleviate pain. That main compounds in Lamiophlomis rotata (Benth) Kudo were reported as flabonoids, iridoids and phenethanol glycoside, but the active components reflecting clinical effectiveness were still unkown. 70% ethanol extraction of the herb, collected in Shiqu county of Sichuan province, was partitioned into four fractions as petroleum spirit, acetic ether, n-butanol and water portion. Guide with initial pharmacological activities test on mice streching model and broken tail model, n-butanol fraction was showed to have obvious analgesic action on reducing the times of mice writing reaction caused by acetic acid and reducing the times of bloodsheding. The portion of water, 30% and 90% ethanolic elution obtained after purification with macroporous resin from n-butanol fraction. 30% of them was found to show strong stanching blood and analgesia activity. Effective in the study were based on the preparation to be effective parts of the mainly Duyiwei intermediates. To improve the preparation Duyiwei bioavailability, and reduce use of volume, We intermediates for the preparation of study materials Duyiwei pills, and to establish the quality standards. Key words: Duyiwei Effective parts 声 明 硕士学位论文 独一味有效部位筛