响应面法优化藏药绿萝花总黄酮的超声波辅助提取工艺-中国药房.PDF

响应面法优化藏药绿萝花总黄酮的超声波辅助提取工艺 1＊ 2 杨小军 ，丁永辉 （1.西北民族大学化工学院，兰州 730124；2.甘肃省食品药品监督管理局，兰州 730070） 中图分类号 R284.2 文献标志码 A 文章编号 1001-0408（2015）25-3565-04 DOI 10.6039/j.issn. 1001-0408.2015.25.36 摘 要 目的：优化藏药绿萝花总黄酮的超声波辅助提取工艺。方法：通过单因素试验考察超声波法提取过程中的时间、温度、乙 醇体积分数、液料比和功率对超声波法提取藏药绿萝花总黄酮提取率的影响；在此基础上，固定超声功率进行4因素5水平的 Box-Behnken 中心组合设计，采用响应面法分析4个因素及其交互作用对总黄酮提取率的影响，确定最优因素及水平并进行验证 试验。结果：最优超声提取工艺为超声提取时间29min 、提取温度44 ℃、乙醇体积分数78％、、液料比13∶1（ml/g ）；3次验证试验 中总黄酮的平均提取率为2.130％，与理论值的相对误差为0.005％。结论：优化工艺合理可行，可用于藏药绿萝花总黄酮的超声 提取。 关键词 藏药绿萝花；总黄酮；超声波辅助提取；Box-Behnken 中心组合设计；响应面法；工艺优化 Study on Optimization of Ultrasonic-assisted Extraction Technology for Total Flavonoids from Tibetan Scindapsus aureus by Response Surface Method 1 2 YANG Xiao-jun ，DING Yong-hui （1.School of Chemical Engineering ，Northwest University for Nationalities ， Lanzhou 730124，China ；2.Gansu Food and Drug Administration ，Lanzhou 730070，China ） ABSTRACT OBJECTIVE ：To optimize the ultrasonic-assisted extraction technology of total flavonoids from Tibetan Scindapsus aureu. METHODS ：The single-factor test was adopted to investigate the effects of extraction time ，extraction temperature ，the vol- ume fraction of ethanol ，the ratio of liquid to solid and power on the extraction rate of total flavonoids from Tibetan Scindapsus au- reu in the process of ultrasonic-assisted extraction ，based on which ，four-factor and five-level Box-Behnken central component de- sign was performed with unchanged ultrasonic power ，and then response surface method was adopted to analyze the effects of the 4 factors and their interaction on the extraction rate of total flavonoids to determine the optimal factor and level ，and verification tests were conducted.RESULTS ：The optimal ultrasonic-assisted extraction technology was as follows as extraction time of 29min ，tem- perature of 44 ℃，volume fract