载有抗逆性基因的多枝赖草染色体连锁群的特定TAC克隆的鉴定-OALib.PDF

华北农学报·2008, 23 (5):6-12 TAC 1, 2 2 1, 2 3 张　艳 ,赵茂林, 孙　淼 ,徐粤宇 (1. , 　100037;2. , 　100097;3., 　411104) 　　: Line24、Line15、Line14 , () 6 SSR , (TAC), 77 , 3 ()()6 SSR , TAC 5 ～ 19 , 13 。Line24 2 TAC 15J18 09I02, (Double-color FISH), 。。 :;TAC ;();; :Q781;Q243;S812.8　　:A　　:1000-7091(2008)05-0006-07 Identification of the Transformation-competent Artificial Chromosome(TAC) Clones Specific to the Linkage Group of Chromosomes Containing Stress-tolerant Genes of Ley mus multicaulis 1,2 2 1, 2 3 HANG Yan , HAO Mao-lin , SUN Miao ,XU Yue-yu (1.Capital Normal University,Beijing 　100037,China;2.Beijing Academy of Agriculture and Forestry Science,Beijing 　100097,China;3.Hunan Institute of Engineering,Xiangtan　411104,China) Abstract:Leymus multicaulis (2n=4X=28,XmXmNsNs)is one of benefit native grass species in Xinjiang,which has many excellent traits such as immune or highly resistant to BYDV (Barley yellow dwarf virus), and highly tolerant to salt,drought,leanness, pollution, aphids and so on.So it is very important to isolate and clone genes controlling these traits in Leymus multicaulis .In this research, the transformation-competent artificial chromosome (TAC)genomic libraries of Ley mus multicaulis were screened through the method of colony blot hybridization in two steps with the probes of spe- cific SSR molecular markers from stress-tolerance chromosomes,which contained stress-tolerant genes of Leymus multi- caulis respectively added to wheat alien disomic addition line.Line 24,tolerant to BYDV and aphids;Line 15,tolerant to salt and drought,and wheat alien disomic translocation-addition line;Line 14