抗氮营养阻遏黄孢原毛平革菌pcR5305产酶条件优化-三明学院学报.PDF

2014年 12月 三 明 学 院 学 报 Dec.2014 第31卷 第6期 JOURNAL OF SANMING UNIVERSITY Vol.31 No.6 凿燥蚤院员园援员源园怨愿辕躁援糟灶猿缘原员圆愿愿辕 扎援圆园员源援园6援园17 抗氮营养阻遏黄孢原毛平革菌 pcR5305产酶条件优化 叶友贤 渊漳州市辐射环境管理站袁福建 漳州363000冤 摘要:对选育的抗营养阻遏产酶黄孢原毛平革菌pcR5305 菌株的产酶营养基质与环境条件进行了优化遥实验结 2+ 果表明pcR5305在37益尧初始pH4.5条件下袁以酒石酸铵(富氮2.2g/L)袁葡萄糖渊20g/L冤袁Mn 渊0.2g/L冤袁Tween 80渊1g/ L冤为主要成分的优质培养基中培养24h后添加5.2mmol/L苯甲醇袁pcR5305 的产酶活性最高遥上述条件下袁该菌接种 后静置培养6 d袁LiP活力可达到2013.59U/L袁MnP活力达到353.42U/L,分别提高了23.41%和27.97%遥 关键词:黄孢原毛平革菌曰木素酶曰抗营养阻遏曰产酶优化 中图分类号院Q939.9 文献标志码院A 文章编号院1673-4343渊2014冤06-0087-06 Optimizing Enzyme-production Conditions ofPhanerochaete chrysosporium pcR5305 which Canbe Resistant to Nitrogen Nutritional Repression YE You-xian (ZhangzhouRadiation Environmental Management Station, Zhangzhou 363000,China) Abstract Weoptimizedtheenzyme-productionnutritionalmediumandenvironmentconditionofthebreedingstrain of Phanerochaete chrysosporium pcR5305 which can be resistant to nitrogen nutritional repression. Results show that pcR5305hadthebestenzymeactivitywhenitwasgrownatthefollowingcondition:themediumwasmixedwithammoni- 2+ umtartrate2.2g/L,glucose20g/L,Mn 0.2g/LandTween801g/L,andaddingbenzylalcohol5.2mmol/Lafter24h,thecul- turingtemperaturewas37℃andtheinitialpHwas4.5.After6dstaticcultured,theLiPactivitycouldreached2013.59U/L, andtheMnPactivitycouldreach353.42U/L,increasedrespectivelyby23.41%and27.97%againsttheun-optimizedcul- turedstrains. Key words Phanerochaete chrysosporium曰ligninolyticenzymes曰resistanceofnutritionalrepression曰