本文通过VC进行软件开发利用数字图像处理技术对菌落图像进行处理与分析.doc

ABSTRACT ABSTRACT PAGE \* MERGEFORMAT II PAGE \* MERGEFORMAT 2 摘 要 PAGE \* MERGEFORMAT I 摘要 对菌落图像进行分析计数是农业、食品、医药卫生分析中进行质量检测的一项很重要的工作。针对菌落图像的特点，本文通过VC++进行软件开发，利用数字图像处理技术对菌落图像进行处理与分析。主要完成了以下工作： 用最大类间差法对采集的菌落图像进行二值化； 通过霍夫变换的方法实现了培养皿边缘的去除，提出了一种改进的椭圆霍夫变换，该方法能很好地抑制噪声和干扰的影响； 通过距离变换法和分水岭算法对粘连菌落进行分割； 通过八领域边缘跟踪和区域填充法对菌落进行标号后计数。 关键词：菌落分析，距离变换，粘连分割，边缘跟踪 ABSTRACT PAGE \* MERGEFORMAT 3 ABSTRACT IMAGE PROCESSING AND ANALYSIS OF COLONY is an important work in Quality Inspection for analysis in the field of Agriculture, Food Industry and medical health industry. According to the feature of the colony image, this article use VC++ to develop software, use digital image processing to analysis the colony image. The main results are as follows: 1. It do binary Colony image by OTSU 2. It realized removing the edge of the dish by the Hough transform method; put forward an improved elliptical Hough transform, which can inhibit noise and the influence of interference. Studied the partition of overlapping colony using chain code deviation theory and a watershed transformation respectively. Eight-connected edge t racking algorithm and area filling algorithm are used to mark and count the colony. Key words: colony counting; distance transform; overlapping partition; edge tracking 目录 目录 目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc295400039 第1章 引言 PAGEREF _Toc295400039 \h 1 HYPERLINK \l _Toc295400040 1.1 国内外研究现状 PAGEREF _Toc295400040 \h 1 HYPERLINK \l _Toc295400041 1.1.1 图像处理在计数方面的应用 PAGEREF _Toc295400041 \h 1 HYPERLINK \l _Toc295400042 1.1.2 菌落分析的发展 PAGEREF _Toc295400042 \h 1 HYPERLINK \l _Toc295400043 1.1.3 粘连菌落分割研究 PAGEREF _Toc295400043 \h 2 HYPERLINK \l _Toc295400044 1.2 研究的目的和意义 PAGEREF _Toc295400044 \h 3 HYPERLINK \l _Toc295400045 1.3 本文主要研究内容 PAGEREF _Toc295400045 \h 4 HYPERLINK \l _Toc295400046 第2章 关于数字图像处理的理论基础及常用处理方法 PAGEREF _Toc295400046 \h 6 HYPERLINK \l _Toc295400047 2.1 数字图像处理的起源及发展 PAGEREF _Toc295400047 \h 6