草莓灰葡萄孢拮抗菌株的筛选、鉴定及其抗菌活性物质研究-生物化学与分子生物学专业论文.docxVIP

1I 1I 问川师范大学硕 1:论文 酸，不含脂基和糖基。 以实验室所保存的多种病原菌为靶标，检测抗菌蛋白 LP18 抗菌活 性。结果表明，抗菌蛋白 LP18 对水稻稻瘾病菌 (Magnαporthe grise α 人 米弯胞病菌 (Curvu/aria /unata) 等多株植物病原真菌均有较强拮抗 活性，具有防治多种植物真商病害的应用前景。 经抗菌蛋白 LP18 作用后的灰葡萄子包菌 (Botrytis cinerea) 显微观 察结果显示，其抗商机理可能是通过溶解细胞撞及改变细胞膜避性导 致原生质渗漏，从而使菌丝断裂或畸形，问时抑制袍子的萌发。 关键词 z 鉴定，枯草芽抱轩菌，抗商蛋白，纯化 网川师范大学硕 网川师范大学硕 1:论文 PAGE PAGE III Screening and Identification of Antagonistic Baeteria against Botrytis cinerea and Study of 、. its Active Substances Major: Biochemistry and Molecular Biology Graduate: Xiong Yan Supervisor: Zhang Hong and Zhang xiaoyu Abstract: A strain Loq18 was screened from soi1 sample of Longquan ， which could strongly antagonize to Botrytis cinerea from Strawberry，by di1ution method.Loql8 was identified as Bac i/lus subtilis through morphological ，physiologic 时， biochemical and molecular identification. Virulence equation of the anfungal activity of fermentation product of Loq 18 to Botrytis cinerea from strawberry was drawn ，we obtained its minimum inhibitory concentration is 20mg/m L. The crude protein with anfungal activity obtained by ammonium sulfate precipitation from sterilization broth ，and the remaining part have none. In order to determine the physical and chemical properties of the antifungal substances produced by Bacillus subtilis strain Loql8 ，the effects of temperature ，pH values ，ultraviolet ，organic solvents ，and protease were studied. The results showed that this crude antifungal substances were thermal stable，about 98% of the initial activity remained after heating at 121.C for 20 min; they were not sensitive to acidity-alkalinity ，in the pH range of 4-11were stable; the antifungal activity was almost not be destroyed when they were under UV irradiation forl0h; when they were treated by ether and acetone ，their inhibition actívity remained above 90% 问川师也大学硕士论文 问川师也大学硕士论文 of control; they were not sensitive to protease. The Sephadex G-75 column chromatography，DEAE Sepharose Fast Flow