层状薄膜的组装及其生物相容性研究化学分析化学专业论文.docxVIP

曲阜师范大学硕士学位论文 曲阜师范大学硕士学位论文 II II Abstract The layer-by-layer assembly (LbL) of sequentially adsorbed, alternating polyelectrolytes has become increasingly important over the past two decades. The ease and versatility in assembling polyelectrolyte multilayers (PEMs) has resulted in numerous wide ranging applications of these materials. Current research and emerging ideas to functionalize surfaces for biological applications will benefit from the unique and highly tunable nature of PEMs as they can be deposited on any surface with a wide range of mechanical and chemical properties. The purpose of this paper is to study the biocompatibility and functionality of the PEMs modulated chemical and physical properties by changing assembly conditions. At first, the interaction of NIH-3T3 fibroblasts with polyelectrolyte multilayer films assembled from PAH and PSS in different salt concentrations with LbL technique were investigated systematically. NIH-3T3 cell adhesion and organization of focal adhesions in cell were evaluated detailedly and quantitatively. The results demonstrated the dependence of cell adhesion on the swelling properties and hydration of the polyelectrolyte multilayer films assembled in different salt concentrations. In this work, by manipulating assembly conditions, such as salt concentration, the molecular architecture and physicochemical properties of the film may be tuned and thus cell adhesion behaviors were tuned. This could be highly potential in designing desired biomaterials for tissue engineering and regenerative medicine. It is also important for PEMs to simulate cellular proliferation and differentiation in addition to facilitating cell adhesion, and to this end we next focused on the development of multilayer films to adsorb and stabilize growth factors initially for delivery to mesenchymal stem cells (MSCs). The adsorption and bioactivity of bone morphogenetic protein 2 (BMP-2) in the films were explored. The response of MSCs to the adsorbed BM