栽培金银花非腺毛性状特征的研究.docVIP

栽培金银花非腺毛性状特征的研究 　　[摘要]采集17个地区22个金银花栽培居群，通过扫描电子显微镜观察和测量花蕾非腺毛的性状特征，利用长度和密度的数据对22个栽培居群进行PCA主成分分析和相关性分析，找出不同产地金银花非腺毛的差异和相关性。结果表明，花蕾非腺毛长度与密度具显著负相关关系，非腺毛密度可将鸡爪花系与毛花系分开，而花蕾非腺毛长度和密度与产地的经纬度没有显著相关性，其中的3个离群居群产生了明显的变异分化可能与气候和土壤的差异有关，暗示栽培金银花花蕾非腺毛的性状特征受环境和遗传共同影响。以上结果为金银花栽培种质和产地鉴别提供了依据。 　　[关键词]金银花;非腺毛;扫描电镜;相关性分析 　　[收稿日期]2014-09-12 　　[基金项目]国家杰出青年科学基金项目;中医药行业专项（201407003）;国家自然科学基金项目 　　[通信作者]*袁媛，副研究员，E-mail：yyuan0732@Study on characteristics of non-glandular hairs of 　　cultivated Lonicera japonica 　　ZHANG Shan-shan1，2， YUAN Yuan1*， HUANG Lu-qi1， CHEN Ping2 　　（1.State Key Laboratory of Dao-di Herbs， National Resource Center for Chinese Materia Medica， Chinese Academy of 　　Chinese Medical Sciences， Beijing 100700， China; 2.Wuhan Polytechnic University， Wuhan 430023， China） 　　[Abstract]We collected 22 cultivated population of Lonicera japonica from 17 areas. The characteristics of non-glandular hairs were observed and measured by the scanning electron microscopy. The principal components analysis and correlation analysis were conduct based on length and density of L. japonica. The results showed a significant negative correlation between length and density of non-glandular hairs， and the characteristics of non-glandular was not corrrelated significantly with latitude. The correlation results indicated that the density was a key to separate ″Damaohua″ and ″Jizhuahua″. The contribution of climate and soil was important to the cultivated population. This reminded that the characteristics of non-glandular hairs were affected by environmental and genetic interaction. 　　[Key words]Lonicera japonica; non-glandular hairs; scanning electron microscopy; correlation analysis 　　doi：10.4268/cjcm 　　药材金银花为忍冬科植物忍冬Lonicera japonica Thunb.的干燥花蕾或带初开的花lt;supgt;[1]lt;/supgt;，具有清热解毒、凉散风热之功效。除黑龙江、内蒙古、宁夏、青海、新疆、海南和西藏无自然生长外，忍冬在我国大部分地区均有分布lt;supgt;[2]lt;/supgt;。作为一种常用大宗中药材，金银花（忍冬）的栽培历史悠久，传统上认为河南新密、山东平邑为金银花的道地产区。目前，除传统道地产区外，其他地区也大面积种植金银花，出现了同一种质在不同地区的性状特征发生变化的现象lt;supgt;[