发酵法生产医用级透明质酸的工艺研究-生物工程专业论文.docxVIP

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发酵法生产医用级透明质酸的工艺研究-生物工程专业论文.docx

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发酵法生产医用级透明质酸的工艺研究-生物工程专业论文

目录中文摘要···································4 英文摘要···································5 第一部分前言································7 1．透明质酸的结构与理化性质 ························7 1.1 透明质酸的结构····························7 1.2 透明质酸的理化性质··························7 2．透明质酸的用途 ·····························8 2.1 透明质酸的生理功能··························8 2.2 透明质酸在化妆品行业中的应用·····················9 2.3 透明质酸在医学上的应用························10 2.4 透明质酸的市场价值··························12 3．透明质酸的制备及生产 ··························12 3.1 已开发的 HA 制备方法及其比较 12 3.2 发酵法生产透明质酸的现状·······················14 4．透明质酸生产的展望 ···························18 第二部分材料与方法 ·····························20 1.试剂···································20 2.仪器···································20 3.菌种与培养基·······························21 4.培养方法·································21 5.分离步骤·································21 6.测定方法·································22 6.1 透明质酸含量测定···························22 6.2 透明质酸特性粘度的测定························23 6.3 蛋白含量的测定····························24 6.4 杂质检测·······························25 6.5 红外光谱·······························26 6.6 紫外光谱·······························26 第三部分结果与讨论 ·····························27 1．HA 的发酵工艺······························27 1.1 发酵温度对 HA 发酵产量的影响 27 1.2 通气量对发酵的影响··························28 1.3 补糖对发酵的影响···························28 2．发酵液的预处理 ·····························30 2.1 乙醇稀释过滤沉淀处理法························30 2.2 超滤处理法······························30 2.3 预处理方法的比较···························32 3．HA 的分离与纯化 32 3.1 乙醇沉淀中氯化钠用量·························32 3.2 乙醇沉淀中 HA 的浓度 33 CTAC 络合沉淀 34 CTAC 残留量 35 4．工艺流程·································35 5．HA 成品的化学分析 37 5.1 透明质酸的红外光谱检测 ························37 5.2 透明质酸的紫外光谱检测 ························38 小结 ·····································40 参考文献 ···································42 摘要透明质酸（HA）是一种广泛存在于人和动物体各组织中的酸性粘多糖，具有重要的生理功能，已被广泛的应用于医药及化妆品等领域。目前我国医用级 HA 的生产主要是通过组织提取法获得，其产量已不能满足市场对 HA 需求的日益增长，而生物发酵法生产药用级透明质酸，具有不受原料资源限制、生产成本低、