狭叶薰衣草特征指纹图谱的研究.docVIP

狭叶薰衣草特征指纹图谱的研究 　　摘要：目的 建立20批狭叶薰衣草的指纹图谱。方法 采用Phenomenex ODS-A色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈和0.036 mol/L磷酸二元梯度洗脱，流速1.0 mL/min，柱温30 ℃，检测波长350 nm。通过相似度评价和主成分聚类分析对20批狭叶薰衣草进行归类。结果 提取了10个色谱峰为狭叶薰衣草的指纹图谱共有峰，指认了2个色谱峰。20批狭叶薰衣草的相似度在0.9以上，通过主成分聚类分析将20批狭叶薰衣草分为3类。结论 该方法操作简便，重复性好，建立的狭叶薰衣草指纹图谱可用于规范和控制狭叶薰衣草药材的质量。 　　关键词：狭叶薰衣草；高效液相色谱法；指纹图谱；迷迭香酸；主成分聚类分析 　　DOI：10.3969/j.issn.1005-5304.2015.04.024 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2015）04-0087-05 　　Study on HPLC Characteristic Fingerprints of Lavandula Angustifolia LI Chen-yang， TAN Wei， CHEN Yan， ZHAO Jun， XU Fang （Xinjiang Institute of Materia Medica， Urumqi 830004， China） 　　Abstract：Objective To establish the fingerprints of 20 batches of Lavandula Angustifolia by HPLC. Methods The determination was performed on a Phenomenex ODS-A column （250 mm× 4.6 mm， 5 μm）. The mobile phase was in gradient elute mode with a mixture consisting of acetonitrile and 0.036 mol/L phosphate acid solution. The flow rate was 1.0 mL/min. The temperature was 30 °C. The determine wavelength was 350 nm. The fingerprints of 20 batches of Lavandula Angustifolia were compared and classified by similarity evaluation， cluster analysis， and principal composition analysis. Results Totally 10 chromatographic peaks were extracted as the common peaks of Lavandula Angustifolia， and 2 peaks were identified. The similarity degrees of the 20 batches of Lavandula Angustifolia were above 0.9. All the batches of Lavandula Angustifolia were classified into 3 categories. Conclusion The method is simple and reproducible， and can be used for the standardization and quality control of Lavandula Angustifolia. 　　Key words：Lavandula Angustifolia；HPLC；fingerprint；rosmarinic acid；principal composition cluster analysis 　　狭叶薰衣草Lavandula angustifolia Mil.为唇形科植物，20世纪初，我国从国外引种栽培成功，现已在新疆伊犁地区大面积推广种植[1-3]。狭叶薰衣草作为新疆特有的芳香类植物，无论在药理活性还是临床应用上都有很高的价值[4-6]。为进一步提高狭叶薰衣草药材质量的可控性，我们在对狭叶薰衣草中迷迭香酸含量测定的基础上，以迷迭香酸为对照建立狭叶薰衣草的标准指纹图谱[7]。本研究考察20个不同产地及批次的狭叶薰衣草，结合中药色谱指纹图谱相似度评价系统进行分析，并建立狭叶薰衣草标准指纹图谱，通过 　　基