超声辅助提取贯叶连翘总黄酮工艺的研究.docVIP

超声辅助提取贯叶连翘总黄酮工艺的研究 　　【摘要】 目的:筛选超声法提取贯叶连翘总黄酮的最佳工艺条件。方法:采用均匀设计法考察乙醇浓度、乙醇用量、提取时间、提取温度、超声提取次数等5个因素对贯叶连翘总黄酮提取的影响;利用紫外-可见分光光度法测定总黄酮的含量;以贯叶连翘总黄酮的得率为指标进行工艺条件的优化。结果:最佳提取工艺为乙醇浓度60%,乙醇用量20 ml/g,每次超声提取时间43 min,提取温度52 ℃,超声提取3次。结论:该提取工艺省时节能,可为工业化生产提供理论依据。 　　【关键词】 均匀设计; 超声法; 贯叶连翘; 总黄酮; 提取 　　Optimization of the Extraction Process of Total Flavonoids from Hypericum Perforatum Based on Uniform Design/ZHU Dong-qing, KONG Qing-xin.//Medical Innovation of China,2012,9(33):151-153 　　【Abstract】 Objective: To optimize the extraction condition of total flavones from Hypericum perforatum by ultrasonic wave. Method: The uniform design method was applied to investigate the effects of alcohol content, the quantity of solvent, extraction time, extraction temperature, and the operation times on the result of extraction. The content of total flavone of Hypericum perforatum was determined by ultraviolet-visible spectrophotometry. The extraction conditions were optimized with the extraction yield of total Hypericum perforatum flavone as index. Result: The optimal extraction conditions were as follows: extracting with 60% alcohol, the quantity of solvent 20 ml/g, extraction time 43 minutes, temperature at 52 ℃, extract for 3 times. Conclusion: The extraction process is reasonable and feasible, and it provides theoretic basis for the industrialized production. 　　【Key words】 Uniform design; Ultrasonic wave extraction; Hypericum perforatum; Total flavonoids; Extraction 　　First-author’s address: Jiangsu Food Science College, Huai’an 223003, China 　　doi:10.3969/j.issn.1674-4985.2012.33.096 　　心脑血管疾病和抑郁症是目前危害人类健康的两类主要疾病[1-2],中医药在治疗心脑血管疾病和抑郁症方面有良好的疗效[3-4]。贯叶连翘(Hypericum perforatum L.)又名贯叶金丝桃,为藤黄科金丝桃属多年生草本植物,是一味古老的传统中药,具有疏肝解郁、清热解毒、收敛止血、调经通乳、利湿消肿之功效[5-6],在我国和欧洲民间都有悠久的药用历史[7]。现代研究表明,贯叶连翘主要有效成分包括苯并二蒽酮类化合物、间苯三酚类化合物和黄酮类化合物等,其黄酮类化合物具有抗炎、抗病毒、抗肿瘤等多种生理活性,可用于防治心脑血管疾病[8-9]。贯叶连翘总黄酮常用的提取方法有水提取法、碱液提取法、醇回流法、醇渗漉法、浸渍法、超声辅助萃取法等。本文以贯叶连翘总黄酮得率为指标,筛选了超声辅助提取贯叶连翘总黄酮最佳的提取工艺条件,可为工业化生产提供理论依据。 　　1 仪器与试药 　　JYC-LQ型超声波萃取仪(上海矩源机械设备有限公司),U