转双价双Bt基因抗虫棉栽培地生存竞争能力的研究.docVIP

转双价双Bt基因抗虫棉栽培地生存竞争能力的研究 　　摘要 为建立国家转双价双Bt抗虫基因棉环境安全评价技术，对转双价双Bt基因（Cry1Ac+Cry2Ab）抗虫棉栽培地生存竞争能力进行研究，结果表明，转双价双Bt基因棉花与对照赣棉11相比，各生育期株高生长具有显著竞争优势，优势值3.48～4.75 cm；吐絮期棉覆盖度竞争优势超对照46.77个百分点，达显著水平；产量竞争力优势籽棉超对照1 013.85 kg/hm2，皮棉超对照433.20 kg/hm2，均未达到显著水平；发芽率竞争优势极显著，超过对照2.75个百分点。与非转基因常规棉相比，转双价双Bt抗虫基因棉生存竞争能力优势显著。 　　关键词 转双价双Bt基因棉；生存竞争能力；生育期；株高；覆盖率；产量；发芽 　　中图分类号 S562 文献标识码 A 文章编号 1007-5739（2012）03-0078-02 　　 　　Study on Survival Competitive Ability of Transgenic Cotton Harboring Cry1Ac+Cry2Ab Double Bt Genes 　　in Cultivated Field 　　ZHANG Xing-hua TIAN Shao-ren ZHANG Tian-yu LI Jie YANG Zhao-guang QIAO Yan-yan 　　（Cotton Research Institute of Jiangxi Province，Jiujiang Jiangxi 332105） 　　Abstract The research studied for establishing the environmental safety assessment technologies of bivalent Bt genes（Cry1Ac+Cry2Ab）transferred cotton in China. The result showed that：the plant height competitive advantage of transgenic cotton harboring double genes（Cry1Ac+Cry2Ab）increased 3.48～4.75 cm in whole growth stages compared to the non- transgenic cotton significantly.The coverage competitive advantage was 46.77 pencentage points more than control significantly.The yield competitive advantage of seed cotton was 1 013.85 kg/hm2 more than control and the lint yield was 433.20 kg/hm2 more than control，but not significantly.The germination rate competitive advantage was 2.75 percentage points more than control，but the difference was not significant. The survival competitive ability of transgenic cotton harboring Cry1Ac+Cry2Ab double Bt genes was higher than the non-transgenic cotton significantly. 　　Key words bivalent insect-resistant Bt genes transferred cotton；survival competitive ability；growth period；plant height；cover rate；yield；germination 　　我国在转基因抗虫棉环境安全评价技术上，仅在2007年公布了1项技术标准，即农业部953号公告-12-2007[1]，这项环境安全评价检测技术标准仅适用于单价转Bt基因抗虫棉，对于双价转基因抗虫棉国内暂缺环境安全评价技术标准。国家农业部从“十一五”开始设立双价转基因棉花环境安全评价技术研究项目。本研究是为转双价双Bt基因抗虫基因（Cry1Ac+Cry2Ab）棉环境安全评价技术的建立提供依据而进行的。 　　1 材料与方法 　　1.1 试验材料 　　供试材料为：转双价双Bt抗虫基因棉花（中国