青壮年猝死综合征脑干中缝背核5羟色胺及5羟色胺转运体免疫组化的研究.docVIP

青壮年猝死综合征脑干中缝背核5羟色胺及5羟色胺转运体免疫组化的研究 　　[摘要] 目的 探讨5-羟色胺和5-羟色胺转运体在青壮年猝死综合征脑组织的表达及其意义。 方法 运用免疫组织化学S-P法检测10例正常脑组织和8例青壮年猝死综合征脑组织中5-羟色胺和5-羟色胺转运体的表达水平，利用图像分析技术测量阳性细胞的平均光密度值。 结果 青壮年猝死征死者脑干中缝背核5-羟色胺的平均光密度值低于正常脑组织，而5-羟色胺转运体的平均光密度值高于正常脑组织，差异均有统计学意义（P＜0.05）。结论 5-羟色胺及5-羟色胺转运体的变化参与青壮年猝死综合征的发病过程，可作为诊断青壮年猝死综合征的参考指标。 　　[关键词] 法医病理学；青壮年猝死综合征；5-羟色胺；5-羟色胺转运体；免疫组化 　　[中图分类号] RF795.1[文献标识码] B[文章编号] 1673-9701（2014）15-0061-02 　　 　　Immunohistochemical study on SMDS dorsal nucleus raphe 5-serotonin and 5-serotonin transporter 　　WU Hua1 TANG Qun2 QU Zhixiong1WANG Jian1 WANG Xinghua1 　　1.Furong Judicial Identification Center of the Second Peoples Hospital of Hunan Province, Changsha 410007,China；2. Department of Pathology of Hunan University of Chinese Medicine, Changsha 410208, China 　　[Abstract] Objective To investigate the 5- serotonin and 5-serotonin transporter expression and significance in brain tissue of sudden manhood death syndrome. Methods Used immunohistochemical S-P staining method in 10 cases of normal brain tissues and 8 cases of sudden manhood death syndrome in expression of 5-HT and 5-serotonin transporter in the brain tissue, using image analysis technique to measure the mean optical density of positive cells of value. Results The average optical density of the 5- serotonin in the dorsal nucleus raphe of sudden manhood death syndrome was lower than that in normal brain tissue, and the average optical density of 5-serotonin transporter was higher than that in normal brain tissue, the differences were statistically significant(P 0.05). Conclusion The changes of 5- HT and 5- serotonin transporter were involved in the pathogenesis of sudden manhood death syndrome, which can be used as a reference marker for diagnosis of sudden manhood death syndrome. 　　[Key words] Forensic pathology; Sudden manhood death syndrome; 5-serotonin; 5-serotonin transporter; Immunohistochemistry本实验通过观察青壮年猝死征（sudden manhood death syndrome ，SMDS）死者脑干中缝背核中5-羟色胺（5-ydroxytryptamine，5-HT）及5-羟色胺转运体（5-HT