去卵巢致大鼠腰椎间盘退变模型初析.docVIP

去卵巢致大鼠腰椎间盘退变模型初析 　　【摘要】 目的：观察去卵巢对大鼠腰椎间盘退变的影响，建立大鼠腰椎间盘退变模型。方法：采用8个月龄健康雌性SD大鼠30只，随机分为空白组、假手术组、模型组，每组各10只。模型组行双侧卵巢切除术，假手术组在其双侧卵巢旁边分别取和卵巢同等大小的脂肪组织，空白组不做任何处理。造模后所有实验大鼠在同等条件下喂养，13周后全部处死，观察其腰椎间盘组织形态学、脊索细胞数量及髓核细胞超微结构的变化。结果：组织形态学评分及脊索细胞数量改变：模型组和空白组比较差异有统计学意义（P0.05）；透射电镜观察其髓核细胞的退变程度：模型组空白组，空白组和假手术组退变程度相当。结论：采用去卵巢的方式能初步建立大鼠腰椎间盘退变模型。 　　【关键词】 腰椎椎间盘退变； 去卵巢； 动物模型 　　A Preliminary Discussion on Lumbar Disc Degeneration Model of Rat by Ovariectomizing/FENG Shuai-hua，WU Guan-bao，YANG Jie，et al.//Medical Innovation of China，2015，12（15）：019-022 　　【Abstract】 Objective：To observe effects of removing ovary on lumbar intervertebral disc， establishing rat model of lumbar intervetebral disc degeneration.Method：8-month-old healthy female SD rats were randomly divided into blank group， sham operation group and model group， 10 rats in each group.The blank group were subjected to no disposal.Rats of the sham operation group only underwent the resection of a bit fat.The model group underwent the resection of bilateral ovaries.all the rats were fed under the same conditions， All rats were sacrificed after feeding 13 weeks， the histomorphology of lumbar disc， notochord cells and the changes of the ultrastructure of nucleus pulposus cells were observed.Result：Tissue morphological grading and notochord cells quantity：there were significant differences between the model group and blank group （P0.05）.Level of degenerative nucleus pulposus cells observed under electron microscope：model group blank group， It had no significant difference between the blank group and the sham operation group.Conclusion：Rat model of lumbar intervetebral disc degeneration was established preliminary through ovary resection of female rats. 　　【Key words】 Lumbar disc degeneration； Ovariectomizing； Animal model 　　First-author’s address：Affiliated Hospital of Hu’nan Traditional Chinese Medicine Institute，Changsha 410060 　　doi：10.3969/j.issn.1674-4985.2015.15.007 　　椎间盘退变引起的腰椎间盘突出、腰椎失稳、滑脱等均是临床上的常见病。国内外关于椎间盘退变的病因和发病机制尚无定论，构