超声造影剂的体内外评价研究-生物医学工程专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 II II Abstract Ultrasound contrast agents (UCAs) improve the contrast of ultrasound images, and can detect the lesions that traditional ultrasound cannot detect, which increases the coincidence rate of diagnosis of hepatocellular carcinoma. At present, the diameter UCAs used in clinical are usually 2~4μm which can pass through the pulmonary circulatory but not the vessel wall, therefore, only a blood pool imaging is possible. It is investigated that tumor vascular endothelial gap will be increased and permits the particles with the diameter less than 700nm pass through. Therefore, the nano-scale UCAs can break through the limitation of blood pool imaging to penetrate into the hepatic tissue and realize an ultrasound molecular imaging which greatly improves the diagnostic sensitivity and specificity, in addition, increases the accuracy of early hepatocellular carcinoma diagnosis. Now the characteristics of nanoscale UCAs are still in the research stage. In this thesis, the properties and contrast effects of nano-scale UCA is investigated by comparing it with SonoVue, a widely used commercial product in China, to establish the foundation for clinical application. In this thesis, the basic characteristics and evaluation in vitro and in vivo of SonoVue and self-made nano-scale UCA are presented. The basic characteristics consist of morphology in macro and micro, diameter distribution and bubble concentration. In vitro, the relationship at the temperature of 37 degrees between UCA concentration and echo signal intensity is addressed by analyzing the enhanced images of SonoVue and self-made nano-scale UCA in the silicone tube to provide basis for the optimal usage of the above self-made UCA. Furthermore, the attenuation of SonoVue and self-made nano-scale UCA is observed. In vivo, the ultrasound images of the rat liver demonstrated that the self-made nano-scale UCA can pass through the pulmonary circulatory to reach the liver, but the