虫生广布拟盘多毛孢GXSTYJ03菌株退化的研究-森林培育专业论文.docx

I I 虫生广布拟盘多毛孢 GXSTYJ03 菌株退化的研究 摘 要 生防菌在生物防治害虫方面起到了举足轻重的作用，有关其退化问 题亦是研究的热点和难点。本文以防治马尾松的主要害虫松突圆蚧的真 菌——虫生广布拟盘多毛孢 GXSTYJ03 菌株为研究对象，探讨了该菌种在 不同环境条件的影响下菌株退化的原因以及退化菌株的复壮途径，旨在 预防 GXSTYJ03 菌株发生退化及菌株退化后所采取的措施，为该菌种在防 治松突圆蚧的研究应用上提供参考依据。本试验结果如下： （1）光照作为真菌生长发育过程中的信息载体，对真菌的生长情 况、次级代谢产物的调节以及菌落局变都有影响。通过不同的光照条件 对虫生广布拟盘多毛孢 GXSTYJ03 菌株退化的影响结果可知，24h 全光照 条件下菌株局变及退化现象均不明显。 （2）培养基营养成分的贫乏是影响菌种退化的主要原因之一。在氮 素适宜的 PPSA1 培养基上虫生广布拟盘多毛孢 GXSTYJ03 菌株退化现象的 发生情况明显减缓，PPSA1 培养基成为防止该菌种退化的较佳培养基，而 且该菌株继代培养次数以 3 代为宜。此外，GXSTYJ03 退化菌株在培养生 长过程中影响并改变了周围培养环境，环境的改变又反作用于该菌株的 生长。 （3）退化菌株恢复原有生物性状的有效手段为复壮。本试验结果表 II II 明虫尸复壮为虫生广布拟盘多毛孢 GXSTYJ03 退化菌株的较好复壮方法， 其中 5%虫尸含量有利于复壮过程中该菌株的生长，7%虫尸含量有利于复 壮时该菌株产孢。 关键词： 虫生广布拟盘多毛孢 GXSTYJ03 环境条件 菌株退化 菌株复 壮 PAGE PAGE IV STUDY ON ENTOMOGENOUS P. DISSEMINATA GXSTYJ03 STRAINS DEGRADATION ABSTRACT Biocontrol fungi has played an important role in biological control of insect pests. So the problems of its degradation are hot and difficult research. The research objected with Entomogenous P. Disseminata GXSTYJ03 which controlled Hemiberlesia pitysophila Takagi in Pinus massoniana. The cause of the strains degradation in the different environmental conditions and the rejuvenation way were studied. There researches aimed to prevent GXSTYJ03 strain degradation and take effective measures after degradation providing a reliable value for application of GXSTYJ03 strain in controlling Hemiberlesia pitysophila Takagi. The main results are as follows: Light as an information carrier in fungal growth and development had played important roles in regulating the fungi growth and secondary metabolism. Moreover，light induced saltation. The result which was study on strain degeneration of Entomogenous P. Disseminata GXSTYJ03 in different light conditions showed that strain degeneration and saltation were not obvious in the full illumination. Nutrient-poor medium was one of the main causes of strain degradation. The PPSA1 medium could slow down and control the degeneration of Entomogenous P