输精管结扎对前列腺细胞凋亡调控的实验与临床分析.pdfVIP

1.1.1.1 12 8.0-14.0kg,11.5kg, 5-6 -1 -1 1.1.1.2 1mg.kg .d 1) 2)HM 340E Microm 3)Leica CM1800 Leica 4)Leica EG1150 Leica 5)Leica DMR+Q550 Leica 1)4%40g0.1mol/L 1000ml pH 7.3 2)PCNABcl-2 Bax SABC Boster 1.1.4.1 1)12 2 2 25% 750mg/kg 2) 12 A B , A 6 0.5%1/3 0.5cm, 1cm 5-0 B 6 3)4% 4 m 60 4h4 HE - PCNA Bcl-2Bax 1.1.4.2 1)AB 12 2)HE 1010 1040 AB 5 60 HE 5min 5min 100% 2min 95%1min 80%1min 75%1min 2min 5min 30s 15min 2min95%1min 95%1min 100% 1min 100%1min 3 11min 1min 1min 3) Leica DMR+Q550 5 1020 4) [8] 1.1.4.3 1)SABC PCNA Bcl-2Bax 5 3% H O 10 min3 2 2 0.02M PBS pH 7.2-7.6 3 5 30 min PCNABcl-2 Bax / 11504 0.02M PBS 2 min3 IgGIgG 37 30 min0.02M PBS 2 min3 SABC37 30 min0.02M PBS 5 min4 DAB 1ml ABC 5-30 PBS PCNA PCNA Bcl-2 Bcl-2 Bax Bax 2)PCNA Bcl-2Bax