蛋白酶表达及细胞迁移影响的实验研究-血液病学专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 4 4 （0.1ng/ml、1ng/ml）and EPO（1IU/ml）+G-CSF（0.1ng/ml、1ng/ml）for 12h, the expression of MMP-2 mRNA was increased. The expression of MMP-2 mRNA was strongest in the EPO(1 IU/ml) combination with G-CSF(0.1ng/ml) treatment group when compared with the control group and single factor group. The expression of MMP-9, TIMP-1 and TIMP-2 mRNA was no difference significantly. ⑤ MSCs were treated with various concentrations of EPO（1IU/ml）、G-CSF （0.1ng/ml、1ng/ml）and EPO（1IU/ml）+G-CSF（0.1ng/ml、1ng/ml）for 12h, the numbers of MSCs invasion was increased. The numbers of cell invasion was maximum in the EPO(1 IU/ml) combination with G-CSF(0.1ng/ml) treatment group when compared with the control group and single factor group. ⑥ MSCs were treated with various concentrations of EPO（1IU/ml）、G-CSF （0.1ng/ml、1ng/ml）and EPO（1IU/ml）+G-CSF（0.1ng/ml、1ng/ml）for 18h, the areas of MSC migration was increased. The areas of cell migration was obvious in the EPO(1 IU/ml) combination with G-CSF(0.1ng/ml) treatment group when compared with the control group and single factor group. ⑦ MSCs were treated with various concentrations of EPO（1IU/ml）、G-CSF （0.1ng/ml、1ng/ml）and EPO（1IU/ml）+G-CSF（0.1ng/ml、1ng/ml）for 3h, ERK1/2 protein was increased. The expression of ERK1/2 protein was strongest in the EPO(1IU/ml) combination with G-CSF(0.1ng/ml) treatment group when compared with the control group and single factor group. Conclusion: EPO combination with G-CSF treatment could promote cell migration by stimulating the expression of MMP-2 on MSC,and probably related to ERK1/2 signal pathway. Keywords: mesenchymal stem cell; erythropoietin（EPO）; granulocyte colony-stimulating factor (G-CSF);matrix metalloproteinase; migration; ERK1/2 独创性声明 本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成 果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文 中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。 学位论文作者签名： 日期： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位