EST-SSR标记开发与莲种质资源遗传多样性分析.pdf

Shanghai Normal University Master of Philosophy Abstract Thesis topic: Development of SSR Markers and Their Utilization in Genetic Diversity Analysis of Nelumbo Professional disciplines: Botany Degree applicant: Yuxian Xu Instructor: Prof. Daike Tian Prof. Jianguo Cao Abstract Lotus （Nelumbo Adands ）is one of the earliest origin taxa in angiosperms. It has a long history of cultivation and diverse cultivars with edible, medicinal, ornamental values. However, classification and genetic relationship on many cultivars remain unclear. The approach only based on the traditional morphology is difficult to accurately identify a cultivar and its relationship with others. In addition, some wild lotus resources are becoming endangered Therefore, scientific evaluation and protective measures should be immediately taken on lotus germplasm. Although some molecular marker s have been recently used to investigate genetic diversity and population relationship in Nelumbo , the application of EST-SSR markers and tag number are yet limited. SSR markers were developed from flower-bud transcriptome sequences of Nelumbo nucifera in this study, and 80 polymorphic EST–SSR markers to evaluate the genetic diversity of the 139 lotus accessions. The main results are as follows: 1. In this study, 68,593 unigenes were assembled from a mixed flower-bud cDNA pool, which was derived from three accessions of N. nucifera to devel