葡萄球菌的快速检测方法研究.docVIP

葡萄球菌的快速检测方法研究 金黄色葡萄球菌(Staphylococcus aureus)在1884年首次被发现，Rosenbach在 固体培养基上培养出葡萄球菌，并在同年记录了它能引起食物中毒。1930年，研宄人员 又明确指出金黄色葡萄球菌引起食物中毒与其产生的肠毒素有关，据估计，进食者摄入 约100ng的肠毒素A即可出现屮毒症状。典型的金黄色葡萄球菌呈球型，直径为0.8 um左右，显微镜下排列成葡萄串状，无芽胞、鞭毛，大多数也无荚膜，属于革兰氏阳 性细菌。目前，随着国际食品贸易不断发展，食品中致病微生物检测方法的研宄也在不 断向前发展，除了传统的培养方法之外，近年来，人们釆用免疫学、生物化学和分子生 物学等先进技术进行了食品中致病微生物快速检测方法的研究。对于金黄色葡萄球菌也 不例外，传统的检测方法主要采用细菌分离培养及生化鉴别，免疫学方法(如：酶联免 疫、免疫转印、乳胶凝集等方法)因其快速、操作简便，逐步应用于食品卫生检测。但 新的分子生物学技术已显示出其在食品卫生检测方面的独特优势。本文主要针对这四种 快速检测方法展开一系列地研究。 Methods of the rapid detection of Staphylococcus aureus ABSTRACT Staphylococcus aureus (Staphylococcus aureus) was first discovered in 1884, Rosenbach in the solid culture medium Staphylococcus， and in the same yearrecorded it can cause food poisoning. In 1930，the researchers pointed out thatdue to Staphylococcus aureus enterotoxin food poisoning and its producing，according to estimates， food intake of about lOOng enterotoxin A can appearpoisoning symptoms. Typical S.aureus is ball shape， diameter of about 0.8 |i m, microscope arranged thyrsoid, non spore forming, flagella， most non capsule， belonging to the gram positive bacteria. At present， with the continuous development of international food trade， pathogenic microorganism detection methods in food is also in constant development， in addition, traditional training methods in recent years, people adopt advanced technology in immunology, biochemistry and molecular biology were studied for the rapid detection of pathogenic microorganism in food. There is no exception for Staphylococcus aureus， traditional detection methods of bacterial isolation culture and identification of the biochemical， immunological methods (such as: ELISA， Western blot, latex agglutination method etc.) because of its fast， simple operation, and gradually applied to the detection of food hygiene. But the new techniques of molecular biology has shown its unique advantages in the aspects of food hygiene detection. This paper mainly launches a series of studies on the four k