橘果皮不同生长期主要活性成分动态变化规律.研究.pdfVIP

为研究对象，采用HPLC 建立了药材中辛弗林的含量测定方法，采用紫外-可见 分光光度计建立了药材中总黄酮含量测定方法，测定了21 批次药材。结果发现 随贮藏时间的延长，广陈皮总黄酮类成分的含量有降低的趋势；辛弗林的含量从 0.273%降至0.1097%，即随贮藏时间的增加有降低的趋势。 关键词：橘果皮 不同生长期 活性成分 动态变化 规律研究 V Abstract The citris peels, Pericarpium Citri Reticulatae (PCR) and Pericarpium Citri Reticulatae Viride (PCRV) ,harvested at different growth stage, are from the Citrus reticulata Blanco and its cultivars of fruit. They are both featured as “regulate qi medicine”, but for the clinical application, PCR has a better effect on regulating qi and strengthening spleen whereas PCRV is strong in soothing liver and breaking qi. As a result from the same plant,PCR and PCRV have some similarities in the terms of active ingredients, efficacy and so on. However, due to the different harvest period, the accumulated changes of secondary metabolites make them different. Current research shows that most of plants secondary metabolites with physiological activity , the medicine efficacy and effect of traditional Chinese medicine (TCM) ,is important efficacy material base. And the synthesis and accumulation of secondary metabolites by their genetic factors restriction and the influence of environmental factors, therefore, are needs to determine origin and plants to study them. Now there is no system to research the composition ,levels and relative proportion of different growth period orange peels secondary metabolites；and Now there is no system to studies on dynamic changes of main Active Ingredient in orange peels of Citrus reticulata blanco from the same plant at different growth periods. So the content of difference between PCR and PCRV in different growth periods,in order to interpret the key factors that affect both drug efficacy differences and provide a theoretic