高表达HOXB4基因对人软骨终板干细胞增殖及细胞周期的影响-外科学(骨外)专业论文.docxVIP

第三军医大学硕士学位论文 万方数据 缩略语表 英文缩写 英文全称 中文全称 LDH intervertebral disc herniation 腰椎间盘突出症 CESCs cartilage endplate stem cells 软骨终板干细胞 HOX homeobox gene 同源盒基因 FBS fetal bovine serum 胎牛血清 HD homeodomain 同源结构域 PCR polymerase chain reaction 聚合酶链反应 Ab antibody 抗体 BSA bovine serum albumin 牛血清清蛋白 d day 日  DMEM/F12 Dulbecco’s modified Eagle’s mediumand Ham’s f-12 改良 Eagle 培养基 G gram 克 h hour 小时 IF Immunofluorence 免疫荧光技术 IHC immunohistochemistry 免疫组织化学技术 min minute 分钟 mRNA messengerRNA 信使 RNA PBS phosphate buffered saline 磷酸盐缓冲盐水溶液 PCR Polymerase chain reaction 多聚酶链式反应  qRT-PCR quantitative real time polymerase chain reaction 实时定量 PCR rpm rotate per minute 每分钟的转速 RT-PCR reverse transcription-PCR 反转录酶聚合酶链锁链反应 sec second 秒 SPSS statistical package for social science 社会科学统计软件包 WB western blot 蛋白质印迹 AGG aggrecan 软骨蛋白聚糖 Col collagen 胶原 1  Effects of HOXB4 over-expression on cell proliferation and cell cycle in human cartilage endplate stem cells Abstract Objectives: To observe the effects of the over-expression of HOXB4 on cell proliferation and cell cycle. Methods: The recombinant over expression vector of HOXB4 was leaded into human cartilage endplate stem cells. The expression of HOXB4 was detected by PCR and Western Blot, CCK8 and FACS analysis were used to determine the effects on cell proliferation and cell cycle respectively. Results: In human cartilage endplate stem cells transfected with the over-expression HOXB4 vector, the expression of HOXB4 was increased notably, and cell proliferation was increased significantly(P0.05). FACS analysis showed that after the over-expression of HOXB4, the population of S phase was decreased from 29.27% to 13.8%(P0.05). Conclusions: HOXB4 over expression in human cartilage endplate stem cells could increase its cell proliferation, decrease the population of its S phase. Therefore, in human cartilage endplate stem cells, HOXB4 may be involved in hindering its degeneration, making it a new study orientation of intervertebral disc degene