膀胱移行细胞癌中整合素连接激酶的表达及意义.doc

PAGE 4 膀胱移行细胞癌中整合素连接激酶的表达及意义 蓝建华,王德林, 杨宗珂, 李 曾 (重庆医科大学附属第一医院泌尿外科，重庆400016) 摘 要: 目的 检测膀胱移行细胞癌(BTCC)和癌旁正常膀胱组织中整合素连接激酶(ILK)蛋白和mRNA的表达，探讨其在膀胱移行细胞癌发生、发展中的作用。方法 利用免疫组织化学S-P法检测56例膀胱移行细胞癌和30例癌旁正常膀胱组织中ILK蛋白的表达，同时运用逆转录聚合酶链反应法检测这些组织标本中ILK mRNA的表达，并与临床病理资料进行相关性分析。结果 56例膀胱移行细胞癌中，ILK蛋白阳性表达率为53.6%(30/56)，ILK mRNA阳性表达率为58.9%(33/56)；而30例癌旁正常膀胱组织中ILK蛋白阳性表达率为10%(3/30)，ILK mRNA表达阳性率为13.3%(4/30), ILK mRNA 和蛋白在癌组织和癌旁正常膀胱组织表达有显著差异(P0.01)。临床病理资料的相关性分析显示，膀胱移行细胞癌中，ILK mRNA和蛋白的表达分布与病理分级有相关性(P0.05)，但与临床分期无相关性(P0.05)。结论 ILK基因转录和蛋白表达可能参与膀胱移行细胞癌的发生过程，ILK可能成为早期发现BTCC的一个肿瘤标志物和治疗BTCC的新靶点。 关键词:膀胱肿瘤； 整合素连接激酶；免疫组织化学；逆转录聚合酶链反应 中图法分类号: 文献标识码: A The expression and significance of Integrin-linked kinase in human bladder transitional cell carcinoma LAN Jian-hua, WANG De-lin, YANG Zhong-ke, LI Zeng (Department of Urology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China) Abstract: Objective To elucidate the role of Integrin-linked kinase (ILK) protein and mRNA in the development of human bladder transitional cell carcinoma (BTCC). Methods The expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and 30 cases of adjacent normal bladder tissue was detected by Immunohistochemistry S-P and Reverse transcription polymerase chain reaction (RT-PCR), respectively. The correlation was analysed statistically, between the expression of ILK protein and mRNA and clinicopathological parameters of human BTCC. Results The positive rate of ILK protein and mRNA in malignant specimens was 53.6% (30/56) and 58.9% (33/56)，respectively, while that in the adjacent normal bladder tissue was 10% (3/30) and 13.3% (4/30), respectively. The expression of ILK protein and mRNA was significantly different between BTCC tissue and adjacent normal bladder tissue (P0.01). The further statistical analysis showed that the ILK protein and mRNA expression was correlated to the pathology grades of BTCC (P0.05), but had no correl