厚皮甜瓜未受精胚珠离体培养技术-蔬菜学专业论文.docxVIP

Invitro舒咀。殍岫ofmelon(Cucun“sraeloL)fromtmpollinatedOV010SAuthor：Han Invitro舒咀。殍岫ofmelon(Cucun“sraeloL)fromtmpollinatedOV010S Author：Han Lihua Major：Olericulture Tutor：Chen Guflin，Wang Jianshe (College ofHorticulture，Agricultural University ofHebei,Baoding,China 171001) The inhibitor of ethylene AgNOj was first used in vitro gynogenesis in melon． Haploid was obtained．Cytological analysis the gynogenesis showed that egg apparatus in embryo developed into embryoid． In late May，ovaries of melon(Cucumis melo L CV．2839)excised 1 4h before anthesis were peeled by hands make the ovules just of the ovary． Longitudinally sliced ovaries were surface sterilized，inoculated pretreatment medium A under 35℃for 4 days in the dark，then transferred induction medium B supplemented withdifferentAgN03 concentration0，5，10，15，20，30，40，60，80，160 mg．L1 fo two weeks under 25。C with 16／8h(1ight／dark)photoperiod．Following the induction period，the ovules we removed from the placenta，transferred elongation and regeneration medium C．Six ovules developed into plantlets by direct embryoids．，Results from the regeneration indicated that 80 mg．L．L AgN03 was favou red the efficiency of gynogenesis，the percentage of embryos reaching 0．74％．By micropropagation and subculture，six regenerants we subdivided into 12 plantlets．Through the number of chloroplasts in guard cell for the ploidy level among the plantlets survived，one is haploid，one is triploid，one is tetraploid，others mixoploid，Cytological observation gynogenesis showed that 14h before anthesis，the embryo have formed the eight nuclei structure，the two polar nuclei still separate each otheL After 4 days heat treatment，some of the gametophytes have proliferated into multi—celled proembryos．After inoculation for 18 days，the shape of the embryoid be clearly in the embryo sacs，showing the embryoid was initiated directly from egg apparatus．In August，inoculation by peeling the ovaries and cutting the ovary into slice，under the identical culture condition