基因工程菌对偶氮染料脱色研究-环境工程专业论文.docxVIP

基因工程菌对偶氮染料脱色研究Abstract 基因工程菌对偶氮染料脱色研究 Abstract Some technologies to treat aZO dye wastewater are in口oduced in this paper，especially the characters and applicmion ofgenetic engineering technology and bioaugmentation．Four aspects are included in the paper，the optimization condition of JMl09①GEX-AZR)fermantation，the the study Oil decolirization ofJMl09(pGEX-AZR)，the study on decolorization ofJMl09，the bioangrnentation oftwo bacteria． The optimal fermentation condition of JMl09(IK3EX-AZR)：Na2HP04(4．269／L)， Ktt2P04(2．659／L)，MnS04’71420(0．0029／L)，FeS04‘7I-120(0．Olg／L)，MgS04+7HzO(0．29／L)， CaCl2(O．029／L)，NH4CI(0．59／L)，Amp．(50rag／L)，glucose(5eeL)，NH4C1(39／L)，pH 7．5， temperature35*C，inoculation qualitylomL／L Under anaerobic condition．JMl09(pGF．X-AZR)Call decolorize more efficiently than aerobic．It can decolorize numerous aZO dyes，such as acid red red B，acid orange II，acid black 10B，acid red G，acid red GR．The decolorization concentration scale is 50～5000mg／L．The optimal decolorizafion condition is pH6～8，25．．40。C，salinity l~5％．The decolorizafion accords with the first class kinetics equation． Under anaerobic condition,E coli JMl09 can decolorize more efficiently than aerobic．It carl decolorize numerous azo dyes，such as acid red B，acid orange II，acid black 10B，acid red G，acid red G1L The decolorizafion rate is 80％at the concentration ofS000mg几．The optimal decolofizationconditionis pH6-g，25--40C，salinity 1--5％． Compared with the activated sludge,the two systems bioaugmented by JMl09(p(3EX- AZR)and E．coli JMl09 I／eat azo dye wastewater more efficiently,especially JMl09(pGEX- AZR)．The optimal glucose concentration of influx is 1000mg／L．JMl09@3EⅪAZR)Can bioaugment the Ire．at ofacid red B，active red K一2BP and acid fuchsin 6B． Key Words：azo dye；genefical engineering microorganism JMl09(pGEX-AZR)； bioaugmentation II 独创性说明作者郑道声明：本硕士学位论文是我个人在导师指镣下进行的研究 独创性说明 作者郑道声明：本硕士学位论文是我个人在导师指镣下进行的研究 工作及取得研究成果。尽我所知，除了文中特别加以标注和致谢的地方 外，论文中不包含其德入已经发表藏撰写的研究成采，也不包含为获得 大连理工大学或其他单位的学位或证书所使用过的材料。与我一同