生长调节剂调控四季蜜龙眼夏季成花机理的初步研究果树学专业论文.docxVIP

根据实时荧光定量结果推测：叶片中DIFTl和DIFT2在PP333和乙烯利诱导 根据实时荧光定量结果推测：叶片中DIFTl和DIFT2在PP333和乙烯利诱导 四季蜜龙眼成花转变的过程中可能起较大作用，且DIFT2的表达量显著上升， 推测DIFT2与成花紧密相关。同时成功构建了过量表达载体pBll21．D1FTl和 pBll21．D1FT2，利用农杆菌介导的花粉管导入法转化拟南芥，收获T0代种子， 为今后基因功能验证以及进一步探明生长调节剂调控四季蜜龙眼夏季成花的分 子机制打下了一定的基础。 关键词：四季蜜龙眼；成花机理；内源激素；F丁基因；表达分析； 载体构建 II 万方数据 Preliminary Preliminary Study on the Flowering Mechanism of Longan cv．Sijimi FhlowerinRg。Summerin ummer RegulatedRegulated withwith Growth Regulators ABSTRACT Longan CV．Sijimi is a tropical ecological type of longan characterizing with multiple flowering annually．Flowering period of the variety could be timed in summer season by means of spring pruning and foliar spraying with growth regulators． But，there have been few reports about the physiological and molecular mechanism of flowering resulting from the treatment．In this study，the change of soluble sugar， starch，soluble protein，free amino acid，N6-(2-Isopentenyl)adenosine、Indolylacetic acid(IAA)、Abscisic acid(ABA)、Gibrellic acid(GA)during the process of flower formation were studied．Besides，the FLOWERING LOCUS T(FT)of Sijimi，the important flowering gene，was achieved by homological cloning and its expressing model and function were analyzed to verify the flowering mechanism of summer flowering of Sijimi longan treated with growth regulators by means of physiology and molecular biology．This study will provide significant theorical guide for flowering and harvesting period regulating of Sij imi longan．The main results of the study were as followings： (1)It was found that foliar spraying with 126．9 mg／L of ethephon+500 m班of PP333 and repeating 7days after with 1 26．9 mg几of ethephon+250 mg／L of PP333 onto S匀iIlli longan trees when their leaves on the fmal shoots of fruiting branch unit became light green in colour and their terminal buds did not germinate improved the amassing flowering of sijimi longan in summer．Study showed that 100％of plant treated flowered and 98．4％of shoots on which carried flower panicles． (2)PP333 and ethep