一种微流控单细胞捕获及生化信号加载装置生物医学工程专业论文.docxVIP

一种微流控单细胞捕获及生化信号加载装置A 一种微流控单细胞捕获及生化信号加载装置 A Microfluidic Device for Single Cell Trapping and Biochemical Signal Loading Ab stract The interactions between biological cells and their extracellular microenvironment have become one hotpot in the research field of cell biology．The manipulation，observation and detection of the single cells cultured in vitro，are the common methods to explore cellular biological behavior．Microfluidic device is not only an ideal experimental platform to capture and manipulate the cells cultured in vitro，but also an important tool to construct exWacellular microenvironments of the cultured cells in vitro．Now it has been widely used in the study of cellular biology．Most of the existing microfluidic—based methods for single·cell trapping have commonly relied on stagnation point flows or the boundary effects of microchannels． The trapping methods with boundary effect were simple，low-cost，and could cap．re a large numbers of single cells with high efficiency，but would fail to easily load external dynamic biochemical signals for the trapped cells．Another kind of trapping methods by stagnation point flows were able to transport biochemical signals but had low stability due to the unsteady stagnation point，which were not convenient to realize long time detection and analysis．Although the introduction with feedback controller may improve the tapping stability，it would need more external control equipment．Thus a microfluidic device for single cell trapping with high stability，inexpensive，and easy-to-use to quantitatively load dynamic biochemical stimuli，is necessary． Based on the principles of hydrodynamics and microfluidics，a microfluidic device for automatically trapping single cell and quantitatively loading dynamic biochemical signal to the trapped single cell was designed using the combination of principle of boundary effect and stagnation point flow in this thesis．The designed micro．device cloud connect with a programmable syringe pump，a microsco