大鼠丘脑腹后内侧核在创伤性脑损伤后电生理变化的实验研究神经外科学专业论文.docxVIP

山西医科大学硕士学位论文The 山西医科大学硕士学位论文 The study on ventral posterior medial hypothalamus of rats after traumatic brain inj ury electrophysiological changes Abstract Object：Electrophysiological nerve cells through experimental observation of traumatic brain mjury and the severity of the medial hypothalamus nuclear abdominal pain-sensitive neurons of the relevance of discharge frequency． Method： 1．Experimental animal and groups：Male Wistar rats 40，weight between 270-2909．Purchased from the Experimental Animal Center of Shanxi Medical University．舢l of rats are randomly divided into four groups．The group of traumatic brain iIljury in accordance with mild， moderate，severe are divided into 3 groups．And other group is sham operated．舻1 o)．We will keep the rats to different cage to eating and drinking． 2．Animal model of TBI：The Feeney method is on basis of falling objects traumatogenesis．Made an impact device which is producted by Instrument factory in academy of military medical Sciences．The headend of strik staffdameter is 4．5ram,height is 2．5ram，peripheral meatus’height is 40cm，maintain vertical 900，Every l cm every pores，to prevent the strik hRmmelwhereabouts of intraductal compression resistance in the air．We USe urethane to anaesth(1．2mg·kg一1)with intraperitoneal injection．After satisfaction of the anaesth，the rat is fixed on stereotaxis instrument．When we make the model of TBI，Cutdown epicranium and undermine periost along the midian line．The incisal opening is 2cm丑ght bregmatic bone Can be exposed．We use dental desk-top electrodill to burr hole with diameter of 5mm which is situated at 1．Smm behind arcuate suture，2．5ram right of linea mediana．We must retain the integ矗ty of cerebral dura mater．Take the head of strik sta行onto the hole，stike hammer(209)will drop down along peripheral meatus with different height 1 0cm and 30cm， we can produce mild and moderate model of cerebral contusion in right parietal．．Anoter, strike hammer(409)drop down from 25cm height， Can produce