不同糖代谢人群胰岛功能与nrf2通路及irs2的关系研究内科学专业论文.docxVIP

T2DM的Nrf2、IRS2水平与NGT比较差异有统计学意义(PO．05)，三组 T2DM的Nrf2、IRS2水平与NGT比较差异有统计学意义(PO．05)，三组 间HO．1、TNF一仅比较差异有统计学意义(PO．0 1)。4．Pearson相关分析显 示Nrf2与HBCI呈正相关(r=0．325，P0．05)，HO．1与HBCI呈正相关 (r=O．299，P0．05)，TNF．仅与HBCI呈负相关(r：一0．462，P0．01)。结论：1． 在糖调节受损时，人体TNF一0【表达升高，提示氧化应激及炎症反应加重。2． 糖尿病前期人群的胰岛功能尚可，此期人体TNF．0【轻度升高，Nrf2、HO一1、 IRS2表达轻度下降，提示Nrf2通路在轻度氧化应激刺激下，表达HO．1 等II相解毒酶以抗氧化应激、炎症损伤，并可能通过P13K／AKT、MAPK 通路与IRS2相关联，保护IRS2顺利转导胰岛素信号通路，以维持胰岛p 细胞数量和功能稳定。3．2型糖尿病患者胰岛功能明显减退，TNF．0t明显升 高，Nrf2、HO．1、IRS2表达明显下降，提示氧化应激及炎症反应加重后， Nrf2通路激活障碍，HO．1表达下降，人体抗氧化能力降低，IRS2降解增 加，胰岛功能受损。 关键词：Nrf2通路；胰岛素受体底物．2；胰岛p细胞功能；氧化应激；炎症 反应；2型糖尿病 万方数据 Part Part one Association of Nrf2 pathway with IRS2 and pancreatic beta cell function：a cross-sectional study Abstract：0bjective：The purpose of this study is to explore the association of Nrf2 pathway with IRS2 and pancreatic beta cell function in people who are type 2 diabetes mellitus(T2DM)，prediabetes(IGR)or normal glucose tolerance．Ultimately，it provides clinical evidence on the prevention and control of beta cell dysfunction． Methods：Based on the epidemiology of evaluation of cancers in Chinese Diabetic Individuals from April to November in 20 1 1,we screened a random sample of 900 individuals into 3 groups：normal glucose tolerance(NGT， n=3 00)，prediabetes(IGR，n=3 00)and newly diagno sed type 2 diabetes mellitus(T2DM，n=300)．All the participants completed a standardized questionnaire，physical examination and biochemical tests．The contents of the laboratory examination included fasting blood glucose(FBG)，2-hours postprandial glucose(2hPBG)，hemoglobin A l c(HbA l c)，fasting insulin (FINS)，triglyceride(TG)，total cholesterol(TC)，high density lipoprotein cholesterol(HDL—C)and low density lipoprotein cholesterol(LDL—C)． Enzyme—linked immunosorbent assay(ELISA)kits were used to measure serum Nrf2，insulin receptor substrate 2(IRS2)，hemeoxygenase 1(HO一1)and tumour necrosis factor一0【(TNF一仅)．Islet function characteristics of different groups were investigated and the association of Nrf2 p