《SNT1196-2003-玉米中转基因成分定性PCR检测方法》.pdfVIP

Protocol of the polymerase chain reaction for detecting genetically modified components in maize T~d$iB.BXj#r#r$iEfB#3;f~HE0 3. 1 RSBB transnene #@N*%A%N,%NSi-F~fbillofllhc~sfi~DNA ~ ~ ~ , ~ @ ~ S C $ I ~ W l ~ % ~ B ~ ? $ : E ~ 3.2 Bbl#JJi$! polymerase chain reaction, PCR ~ ~ J ? ~ J Y i i % ! ~ % l ~ ? k ~DNA~ @ @fiH*M#SHE{$T*@BPJg~ 2-t1W+%~14@3)9II%8$5DNA H%#LfiiW-BX$bIP9rJ2+BA~#X%6,f# DNA Re - m 6 ~ @ r N t m s a ~ ~ L49-~ n l ~ ~ ~ v 3 . 3 HMl% 3 . 3 1 PCR polymerase chain reaction, j%j% PCR. 3 3. 2 DNA,deoxyribonucleic acid,%#+@. 3 3 3 dNTP,deoxyribonucIeos~detr~phosph~te.H#g@f(Z@@. 3 3 . 4 ATP PI deoxyadeNOSine t i . i p h o s p l ~ ~ t e , ~ ~ I B ~ @ @ 3 . 3 . 5 dCTP,deoxycyt~dlnetriphosph~te,a$f.fi#Z#@ a 3 3 . 6 dtiTP,deoxyguaNOSine triphoupliate,n#!P5 83@mZll. 3 3.7 dTTP,deoxythym~dinetr1plrosphate,jttlffw8:ii@@~ 3 3 . 8 dUTP,deoxyurid~netriphosl)hate,MRtiirtfZ#@ 3.3.9 bpl base pair, 4iH. 3.3. 10 EDTAI ethylene diaminetetraacetic a c i d t Z , Z @ ~ Z , ~ . 3.3. 1 1 Tagl Thermu~aquaticu ,!%#!%. 3.3. 12 Trisl tris(hydroxymethyl)aminomethanetFi(~I)$lBfe$2, 3.3. 13 TEITris-C1,EDTA ##a, 3.3.14 IVR~invertaselgenefrommaize,3*@fiBl%m. 3.3.15 Zeinlh*l#@biPBSB$, 3. 3. 16 CaMV 35S135S promoter from cauliflower mosaic virus, fi ?g#%awJi$t#J355 a3. 3.3. 17 NPTIIl neomycin-3-phosphotransferasegenet%#* - 3 - @ @ 8 @ H . 3.3. 18 NOS1 terminator of nopaline synthase gene,861813U9ElSmk+. 3.3. 19 IVS21intron from the maize alcohol dehydrogenase gene,3#B)rB#%#%ElWBJfi E2. 3 . 3 20 PATt phosphinothricin acetyltranrferase gene*PTf#Z,B%##Sfl. 3.3.21