模式动物果蝇对生命科学研究的重要贡献.PPT

Two Most Active Players in the Fly Community in the Last Twenty Years Rubin, G.M., Spradling, A.C. Genetic transformation of Drosophila with transposable element vectors. Science, 1982 218(4570):348—353 Prominent leaders in the Drosophila Genome Project Gerry M. Rubin UC Berkeley Vice President of the HHMI Allan C. Spradling, Director of the Department of Embryology of the Carnegie Institute Gerry: the Berkeley Drosophila Genome Project, sequencing of genomic clones, EST clones and mapping of P element insertions. The genome sequence of Drosophila melanogaster. Science 2000 287(5461):2185--2195 Allan: Genetic screens to mutate the genome by P elements, aiming at having a P insertion in each of the 15,000 genes. The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. 模式动物果蝇在生命科学和医学研究中的应用 1，果蝇在生命科学研究中的历史贡献 2，来源于果蝇研究中的重大技术突破 (P Insertion; UAS-GAL4; FRT-FLP) 3，果蝇在现代医学研究中的应用 Landmarks in Fly Genetics Elucidation of early development by systematic genetic screens, 1980; Nobel Prize in 1995 Germline transformation of fruit flies, 1983 Completion of Genome Sequencing in 2000, fly as a model for human diseases Mutagenesis by P element insertion, each gene has a insertion UAS-GAL4 system to manipulate gene expression FRT-FLP system to make mosaic clones Gene Expression Manipulation Brand, A.H., Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. DEVELOPMENT 1993 118(2):401--415 Rorth, P. A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. PNAS 1996 93(22):12418--12422 Rorth, P., Szabo, K., Bailey, A., Laverty, T., Rehm, J., Rubin, G., Weigmann, K., Milan, M., Benes, V., Ansorge, W., Cohen, S. Systematic gain-of-function genetics in Drosophila. DEVELOPMENT 1998 125(6):1049--1057 UAS-GAL4 System Derived From Yeast Cited: 5983 GAL4–UAS System for Directed Gene Expression GAL4: a transcription factor UAS: upstream activating sequenc